PrimeTaq™ SYBR Green qPCR MasterMix, which premixes reagents including chemically modified PrimeTaq™ HotStart Direct PCR DNA Polymerase, reaction buffer, SYBR Green, and dNTPs. This product is equipped with a separate ROX reference dye, which can be used for inter-well calibration in quantitative PCR instruments that require ROX.
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PrimeTaq™ HotStart Direct PCR DNA Polymerase in this MasterMix has high impurity tolerance and it is extremely resistant to ethanol, guanidinium salts, heparin, serum, plant polysaccharide polyphenols. Therefore, it can be used for direct quantitative PCR detection of crude samples. With hot start property, the polymerase is 100% inactive below 50°C and can be completely recovered only after heating at 95°C for 5 min. Therefore, this system can effectively inhibit nonspecific PCR amplification, greatly improving the specificity of PCR amplification.
Robust amplification performance and can be used for qPCR amplification above 4-plex.
High tolerance to impurities, which makes it ideal for direct PCR.
Highly sensitive and wide range of dynamic detection (> 7 orders of magnitude).
The reaction efficiency is between 95-100% with high accuracy and reproducibility.
The amplification can be completed within 40 min, amplifying 1kb within 10 seconds.
Produce higher fluorescence detection on different AT-rich and GC-rich targets.
The minimum shelf life is 3 years under -20°C with light-free. The performance of the reagent will not decrease after 30 times of freezing and thawing.