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Cat. No.: PAMA-200 (for 200 μl)

Cat. No.: PAMA-1k (for 1 ml)

Description

PrimeAb Mouse Adjuvant (Water Soluble) is used for mouse polyclonal and monoclonal antibody production with high titers. Compared with conventional Freund’s adjuvant, PrimeAb Mouse Adjuvant has many advantages:

• Remove the antigen emulsification step
• Fewer immunization shots
• Lower antigen dosage
• Higher titer
• Higher antibody affinity
• Less damage to the natural conformation of the antigen
• Easy to transport and preserve.

Immunization Schedule

1. Immunogen Dilution: Calculate the required amount of the immunogen, and dilute the immunogen with sterile PBS or SPSS (stroke-physiological saline solution) to 2 times of its final concentration.

Mice: 50-100 μl per injection except for the final boost when 100-200 μl should be used.

Rats: 50-100 μl per injection except for the final boost when 100-200 μl should be used.

2. Take 10 μl adjuvant under sterile conditions and mix with the immunogen at the volume ratio of 1:5-1:10, mix the solution thoroughly.

3. Inject the mice/rat with the immunogen & adjuvant mixture 50 μl subcutaneously.

4. Immunization Timetable:

The 1st Day:

Follow Step 1 to Step 3.

The 10th Day

Repeat the injection. ELISA detection of the serum titer.

The 17th Day

Repeat the injection and bleed. ELISA detection of the serum titer.

The 24th Day

Bleed and Repeat the injection. ELISA detection of the serum titer.

Generally, after the third or fourth injection, the ELISA titer is around 10⁴-10⁷, whole blood can be collected or the antigen impulse immunization and spleen cell fusion can be performed according to the traditional procedure. According to our experimental result, the serum titer (protein immunogen) will peak after 3rd/ 4th injection.

Notification: This product is for research use (RUO) and investigational use only (IUO). IT CAN NOT BE USED ON HUMAN OR DIAGNOSTIC PROCEDURES.

Storage

Store at 4°C for up to 6-12 months. Avoid repeated freezing and thawing.