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Cat. No.: PPGT12-10-10 (for 10 wells, 10 pieces)
Cat. No.: PPGT12-15-10 (for 15 wells, 10 pieces)
Cat. No.: PPGT12-10-50 (for 10 wells, 50 pieces)
Cat. No.: PPGT12-15-50 (for 15 wells, 50 pieces)
Description
Precast PAGE Gel for Tris-Gly System is a new generation precast gel for coating plastic plates. It is a convenient, high-quality, and safe polyacrylamide gel with standard dimensions, designed for use with the Tris-Glycine electrophoresis buffer system. It allows for electrophoresis to be completed in just 40-60 minutes, producing very smooth and sharp bands. This precast gel includes a concentrated gel region of approximately 1.5 cm in height, providing excellent separation results. After electrophoresis, protein bands appear smooth, clear, fine, and sharp, with minimal edge effects. Additionally, this precast gel plate is made of coating plastic material, reducing non-specific protein adsorption, leading to superior electrophoresis performance, even surpassing that of self-made PAGE gels. It is commonly used for PAGE and Western blotting.
Precast PAGE Gel for Tris-Gly System is available in various gradient and fixed concentration gels, with options for 10-well and 15-well plates. Gradient gel concentrations range from 4-15%, 4-20%, 8-16%, and 8-20%, while fixed concentration gels are available in 6%, 8%, 10%, 12%, and 15%. The 4-20% gradient gel is the most commonly used. Customized services are available for special concentration requirements. Please refer to the table below for the optimal separation range of each precast gel:
Precast Gel Concentration | Number of Wells | Maximum Loading Volume | Electrophoresis/Transfer Buffer System | Optimal Separation Range |
---|---|---|---|---|
6% | 10/15 | 50/30μl | Tris-Glycine | 50-150kDa |
8% | 10/15 | 50/30μl | Tris-Glycine | 30-90kDa |
10% | 10/15 | 50/30μl | Tris-Glycine | 20-80kDa |
12% | 10/15 | 50/30μl | Tris-Glycine | 12-60kDa |
15% | 10/15 | 50/30μl | Tris-Glycine | 10-40kDa |
4-15% | 10/15 | 50/30μl | Tris-Glycine | 20-180kDa |
4-20% | 10/15 | 50/30μl | Tris-Glycine | 10-180kDa |
8-16% | 10/15 | 50/30μl | Tris-Glycine | 20-90kDa |
8-20% | 10/15 | 50/30μl | Tris-Glycine | 10-90kDa |
This precast gel contains approximately 1.5 cm of 4% concentrated gel, ensuring the production of very sharp bands effectively.
The ratio of acrylamide to bisacrylamide in this precast gel is 29:1, and the gel thickness is 1.0 mm. The maximum loading volume for 10-well configurations is 50 μl, while for 15-well configurations, it is 30 μl. The dimensions of the gel plate are 100 × 90 × 4.8 mm (width × height × thickness), and the gel size is 84 × 74 × 1 mm (width × height × thickness).
Polyacrylamide gel electrophoresis (PAGE) technology is widely used for protein separation, purification, detection, identification, and molecular weight analysis. It is one of the fundamental techniques in life science research. Common Western blot assays are based on PAGE.
The PAGE precast gel (Tris-Gly) is prepared with near-neutral pH Tris-HCl buffer, does not contain SDS, and can be used for both denaturing and non-denaturing protein electrophoresis.
Features
Storage
Store at 4°C, effective for one year. Do not freeze below 0°C.
Only for research and not intended for treatment of humans or animals
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