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Cat. No.: PBSTQD-100 (for 100×1L) 

Description

PBS-T is a white to off-white quick-dissolve pellet. Each pouch of PBS-T quick-dissolve pellets can be used to prepare 1 L of 1× PBS-T buffer, making it easy and convenient to use. The primary components of PBS-T include sodium chloride, potassium chloride, phosphate, and Tween-20. In 1× PBS-T buffer, the concentration of sodium chloride is 137 mM, potassium chloride is 2.7 mM, phosphate is 10 mM, and the content of Tween-20 is 0.05%.

PBS-T buffer contains a non-ionic detergent, Tween-20, and is widely used in immunological assays such as ELISA and WB. It is commonly used as a washing buffer and for antibody dilution and blocking solutions.

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Components

• PBS-T: 100 Pouches (100×1L)

Protocol

1. Measure approximately 600 ml of distilled water and add it to a beaker. Place a magnetic stir bar inside the beaker.
2. Position the beaker on a magnetic stirrer and slowly add the entire contents of one pouch of PBS-T quick-dissolve pellets. Stir the solution until it is completely dissolved.
3. Add distilled water to the PBS-T solution from step 2, bringing it to a final volume of 1 L, making it 1× concentration.

Storage

Room temperature.

Only for research and not intended for treatment of humans or animals

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