MycoErase™ Mycoplasma Remover
$135.00 - $1,400.00
All products have special prices for bulk purchase, please contact us for more details if required.
Cat. No.: MEMR-50 (for 50T)
Cat. No.: MEMR-250 (for 250T)
Cat. No.: MEMR-1000 (for 1000T)
Description
MycoErase™ Mycoplasma Remover is a non-antibiotic reagent used for the rapid, efficient, and convenient removal of widespread mycoplasma contamination in cell cultures. This product is a truly effective agent that thoroughly eliminates and kills mycoplasma, and it can be removed after treatment without the need for long-term maintenance. It is suitable for the treatment of various mycoplasma-contaminated cells, viruses, and related reagents. It is particularly suitable for mycoplasma removal from valuable cells that are contaminated with mycoplasma or for the preservation of mycoplasma-contaminated cells that need to be maintained.
Features
- MycoErase™ is user-friendly, as no pre-treatment of cells or removal agent is required. Cells can be cultured and passaged normally. Simply replace the cell culture medium with a medium containing this product. The mycoplasma can be effectively removed within 2-5 days with 1-3 applications.
- MycoErase™ is capable of rapidly and effectively eliminating mycoplasma contamination in cell cultures, with a root eradication efficiency of nearly 100%. Most commercially available mycoplasma removal reagents consist of tetracycline, macrolide, or quinolone antibiotics, which primarily exert inhibitory effects on mycoplasma rather than directly killing them. As a result, their action is relatively slow and the effects are not long-lasting. MycoErase™, on the other hand, is predominantly composed of a peptide-based surfactant derived from the fermentation extract of Bacillus subtilis. Based on the principles of microbiology and physical chemistry, MycoErase™ selectively binds to the mycoplasma membrane, altering its permeability and leading to mycoplasma rupture and death. Due to this mechanism, the likelihood of mycoplasma developing resistance is extremely low. After successful mycoplasma eradication, MycoErase™ can be easily removed, allowing the cells to promptly restore their natural morphology and normal proliferation rate.
- MycoErase™ provides rapid action and stable results. It only requires 1-3 treatments, with an effect that lasts for 2-5 days, effectively eliminating mycoplasma from cell cultures for an extended period. Compared to other mycoplasma removal reagents, it is more efficient and faster. Antibiotic-based removal or clearance agents typically require multiple treatments and longer exposure times to effectively clear or inhibit mycoplasma, and they often need to be used for an extended period of time to maintain effectiveness. In contrast, this product only requires 1-3 treatments and can be removed after achieving mycoplasma clearance, without the need for long-term maintenance.
- MycoErase™ does not contain antibiotics, which prevents the development of antibiotic resistance in mycoplasma. It has low cytotoxicity towards cells. The product selectively binds to the mycoplasma membrane, altering its permeability and effectively killing the mycoplasma. Although MycoErase™ may have some impact on cell viability and morphology during the treatment period, it cannot penetrate the cell membrane of eukaryotic cells and therefore does not alter any cell characteristics. After mycoplasma removal, cells can rapidly recover their normal growth and proliferation status during subsequent passages, with almost no impact on their growth and proliferation.
- MycoErase™ is a ready-to-use sterile solution, providing convenient usage. Simply replace the cell culture medium with the medium containing this product, without the need to reduce serum concentration.
Additional Notes
- Although MycoErase™ effectively eliminates mycoplasma from cell cultures, there is still a possibility of recontamination due to incomplete clearance, subsequent handling, reagent contamination, and environmental factors. Therefore, it is recommended to perform mycoplasma testing on a weekly basis during cell culture, as well as promptly after thawing cryopreserved cells.
- MycoErase™ utilizes a biophysical approach to selectively bind to the mycoplasma membrane and eradicate the contamination. Direct contact with mycoplasma is necessary for effective eradication. When dealing with suspension cells, simply replacing the cell culture medium with MycoErase™-containing medium is sufficient. For adherent cells, it is advised to add MycoErase™ when cells are in suspension during digestion and passaging, as this method yields better mycoplasma removal compared to adding the product after cells have adhered. Moreover, this approach minimally affects cell viability and morphology.
- During the action of MycoErase™, there may be some impact on cell vitality and morphology. To ensure proper cell growth following treatment, it is essential to maintain an adequate cell density. For example, a cell confluence of 60% or higher is recommended for adherent cells in a 6 cm culture dish. For suspension cells, it is preferable to have a cell count of at least 300,000 cells. However, excessive cell density may diminish the effectiveness of MycoErase™. Different cell types exhibit varying tolerance levels, so adjustments to the cell count can be made accordingly. Close observation of cell behavior is advised during the treatment period. If cell deformation or slow growth is observed, terminating the treatment by replacing the medium with standard culture medium or diluting the MycoErase™ reagent at a ratio of 1:1500 or even higher can be considered. However, reducing the concentration of MycoErase™ may require additional treatment cycles and prolonged exposure time.
- The time required for mycoplasma-negative conversion after treatment depends on factors such as cell type, cell condition, mycoplasma species, and the severity of contamination. For cells that have achieved negative conversion after MycoErase™ treatment, it is recommended to perform an additional consolidation treatment to ensure thorough mycoplasma clearance and prevent recontamination. For example, in our experiments with MOLM-13 suspension cells, a single treatment for two days resulted in mycoplasma-negative conversion, which remained stable. However, HeLa cells achieved mycoplasma-negative conversion after one treatment but had the potential for recontamination after passaging. In such cases, a second treatment significantly enhanced stability.
- Following mycoplasma elimination with MycoErase™, residual mycoplasma may still be detected. Firstly, during the process of mycoplasma elimination, the mycoplasma membrane dissolves, releasing mycoplasma DNA into the culture medium, which can lead to false-positive results in mycoplasma nucleic acid testing. As cells continue to be cultured, extracellular DNA gradually degrades. Therefore, it is advisable to perform mycoplasma testing 3 to 4 passages after treatment with MycoErase™. Secondly, the efficacy of MycoErase™ can be influenced by factors such as cell density and cell condition. Initial treatment may not completely eliminate mycoplasma, and improper handling can lead to recontamination. In such cases, it is recommended to perform a second treatment after the cell condition stabilizes.
- Prior to mycoplasma removal, it is essential to test the host cell line for mycoplasma contamination to ensure the absence of contamination.
- Enveloped viruses share similar lipid membrane components with mycoplasma, making them susceptible
- to removal by MycoErase™. The effectiveness of MycoErase™ against enveloped viruses depends on the concentration and duration of treatment. To ensure thorough mycoplasma removal while preserving viral infectivity, the initial viral titer should exceed 106 TCID50 (50% Tissue Culture Infectious Dose). In contrast, the removal of non-enveloped viruses is not influenced by viral titer.
- MycoErase™ is intended for use by qualified professionals in scientific research only and should not be used for clinical diagnosis or treatment. It is not intended for use in food or drugs and should not be stored in residential areas.
- For your safety and health, please wear laboratory attire and disposable gloves while handling.
Storage
The storage temperature is 4°C, and the product is effective for one year.
Only for research and not intended for treatment of humans or animals
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