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Cat. No.: MPRMSC-20 (for 20T)

Cat. No.: MPRMSC-50 (for 50T)

Cat. No.: MPRMSC-100 (for 100T)

Description

Muta-Prem™ Random Mutagenesis & Seamless Cloning Kit consists of two components: Random Mutagenesis Module and Single-Fragment Gene Cloning Module. The Random Mutagenesis Module is supplied as a 2× mixture containing low-fidelity Taq DNA polymerase, error-prone DNA polymerase, dNTPs and optimized random mutagenesis reaction buffer. To amplify target DNA, simply add template DNA, primers and nuclease-free water to the 2× Mix to dilute it to a working concentration of 1× prior to PCR setup.

The Single-Fragment Gene Cloning Module enables ligation-independent gene cloning. It delivers high colony yield and high positive clone rate, facilitating straightforward insertion of randomly mutated DNA into target vectors. Detailed protocols for the Single-Fragment Gene Cloning Module are available in the product manual.

Features

• Capable of amplifying target DNA fragments up to 4–5 kb
• Controllable random mutagenesis rate: 1–2 mutations per kb
• Compatible matched Single-Fragment Cloning Module: one-step seamless cloning with high colony number and high positive clone ratio
• Works with standard chemically competent cells; no specially prepared competent cells required

Applications

Controlled random mutagenesis of genes for introduction of random base substitutions

Related:

• Muta-Prem™ Single-Site Directed Mutagenesis Kit
• Muta-Prem™ Chemical-Based Site-Directed Mutagenesis Kit
• Muta-Prem™ Plus Site-Directed Mutagenesis Kit
• Muta-Prem™ Multi-Site Directed Mutagenesis Kit
• Muta-Prem™ Random Mutagenesis Kit
• Muta-Prem™ Random Mutagenesis & Seamless Cloning Kit

Featured Citations

Interested in seeing published research using our Mutagenesis Kits?

NLRP6 potentiates PI3K/AKT signalling by promoting autophagic degradation of p85α to drive tumorigenesis

^{_{Nature Communications    |    28 September 2023    |    DOI: https://doi.org/10.1038/s41467-023-41739-z}}

^{_{p85α mutants were generated using a site-directed mutagenesis kit (SBS Genetech, Beijing, China) according to the manufacturer’s instructions.}}

ZmBSK1 positively regulates BR-induced H2O2 production via NADPH oxidase and functions in oxidative stress tolerance in maize

^{_{Plant Physiology and Biochemistry    |      15 August 2022    |    Doi: https://doi.org/10.1016/j.plaphy.2022.06.011}}

^{_{The mutated ZmCCaMK was obtained using the Site-Directed Mutagenesis Kit (SBS Genetech) followed by the manufacturer's protocol.}}

Rhophilin rho GTPase binding protein 1-antisense RNA 1 (RHPN1-AS1) promotes ovarian carcinogenesis by sponging microRNA-485-5p and releasing DNA topoisomerase II alpha (TOP2A)

^{_{Bioengineered    |      07 Dec 2021    |    Doi: https://doi.org/10.1080/21655979.2021.2002494}}

^{_{The site-directed mutagenesis kit (SBS Genetech, China) was used to mutate the WT binding sequence of RHPN1-AS1 or TOP2A 3ʹ-UTR, and the produced mutant sequence was also inserted into psiCHECK2 vectors, which were named RHPN1-AS1 Mut1, RHPN1-AS1 Mut2, RHPN1-AS1 co-Mut, and TOP2A Mut vectors.}}

Thr420 and Ser454 of ZmCCaMK play a crucial role in brassinosteroid-induced antioxidant defense in maize

^{_{Biochemical and Biophysical Research Communications Supports open access    |      7 May 2020    |    Doi: https://doi.org/10.1016/j.bbrc.2020.02.078}}

^{_{The Site-Directed Mutagenesis Kit (SBS Genetech, China) was used to obtain the site-directed ZmCCaMK according to the manufacturer’s instructions.}}

Long non-coding RNA LOC554202 promotes acquired gefitinib resistance in non-small cell lung cancer through upregulating miR-31 expression

^{_{Journal of Cancer    |     15 Oct 2019    |    Doi: https://doi.org/10.7150%2Fjca.35097}}

^{_{The mutation of miR-31 binding sites in 3'-UTRs of RASA1 (UCUUGCC was mutated to UGUUCGG) or FIH-1 (UCUUGCC was mutated to UGUUCGG) was performed by a Muta Direct Site-directed Mutagenesis kit (SDM-15; Beijing SBS Genetech Co, Ltd, Beijing, China).}}

Long Non-coding RNA H19 Inhibits Adipocyte Differentiation of Bone Marrow Mesenchymal Stem Cells through Epigenetic Modulation of Histone Deacetylases

^{_{Scientific Reports    |     28 June 2016    |    DOI: https://doi.org/10.1038/srep28897}}

^{_{Site-directed mutagenesis of the H19 sequences was performed using the Site-Directed Mutagenesis Kit (SBS Genetech, Beijing, China).}}