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Cat. No.: MPSS-20 (for 20T)

Cat. No.: MPSS-50 (for 50T)

Cat. No.: MPSS-100 (for 100T)

Description

Muta-Prem™ Chemical-Based Site-Directed Mutagenesis Kit enables introduction of specific targeted mutations at any position within plasmid DNA sequences, including base insertions, deletions and base substitutions.

Firstly, wild-type plasmid is amplified by PCR using high-fidelity DNA polymerase and primers carrying target mutations together with phosphorothioate modifications. This reaction linearizes the plasmid and incorporates desired mutation sites. Afterwards, chemical recombination reagent is applied to recircularize the linearized plasmid. The forward and reverse amplification primers are designed to contain 8–12 complementary bases at their respective 5′ termini to support plasmid recircularization via chemical recombination. The linearized PCR products harboring target mutations are treated with chemical recombination reagent for circularization, followed by transformation into DH5α competent cells to complete site-directed mutagenesis.

Our Muta-Prem™ Chemical-Based Site-Directed Mutagenesis Kit contains 2× High-Fidelity PCR Mix and chemical recombination reagent, supporting a complete end-to-end experimental workflow and greatly simplifying operation. A separate version without 2× High-Fidelity PCR Mix is also available.

Feature

• Generate substitutions, insertions and deletions at arbitrary positions on plasmids
• Rely on chemical recombination instead of recombinase protein
• Complete one-stop system including high-fidelity PCR components

Applications

Site-directed DNA mutagenesis, including base substitutions, insertions and deletions

Related:

• Muta-Prem™ Single-Site Directed Mutagenesis Kit
• Muta-Prem™ Chemical-Based Site-Directed Mutagenesis Kit
• Muta-Prem™ Plus Site-Directed Mutagenesis Kit
• Muta-Prem™ Multi-Site Directed Mutagenesis Kit
• Muta-Prem™ Random Mutagenesis Kit
• Muta-Prem™ Random Mutagenesis & Seamless Cloning Kit

Featured Citations

Interested in seeing published research using our Mutagenesis Kits?

NLRP6 potentiates PI3K/AKT signalling by promoting autophagic degradation of p85α to drive tumorigenesis

^{_{Nature Communications    |    28 September 2023    |    DOI: https://doi.org/10.1038/s41467-023-41739-z}}

^{_{p85α mutants were generated using a site-directed mutagenesis kit (SBS Genetech, Beijing, China) according to the manufacturer’s instructions.}}

ZmBSK1 positively regulates BR-induced H2O2 production via NADPH oxidase and functions in oxidative stress tolerance in maize

^{_{Plant Physiology and Biochemistry    |      15 August 2022    |    Doi: https://doi.org/10.1016/j.plaphy.2022.06.011}}

^{_{The mutated ZmCCaMK was obtained using the Site-Directed Mutagenesis Kit (SBS Genetech) followed by the manufacturer's protocol.}}

Rhophilin rho GTPase binding protein 1-antisense RNA 1 (RHPN1-AS1) promotes ovarian carcinogenesis by sponging microRNA-485-5p and releasing DNA topoisomerase II alpha (TOP2A)

^{_{Bioengineered    |      07 Dec 2021    |    Doi: https://doi.org/10.1080/21655979.2021.2002494}}

^{_{The site-directed mutagenesis kit (SBS Genetech, China) was used to mutate the WT binding sequence of RHPN1-AS1 or TOP2A 3ʹ-UTR, and the produced mutant sequence was also inserted into psiCHECK2 vectors, which were named RHPN1-AS1 Mut1, RHPN1-AS1 Mut2, RHPN1-AS1 co-Mut, and TOP2A Mut vectors.}}

Thr420 and Ser454 of ZmCCaMK play a crucial role in brassinosteroid-induced antioxidant defense in maize

^{_{Biochemical and Biophysical Research Communications Supports open access    |      7 May 2020    |    Doi: https://doi.org/10.1016/j.bbrc.2020.02.078}}

^{_{The Site-Directed Mutagenesis Kit (SBS Genetech, China) was used to obtain the site-directed ZmCCaMK according to the manufacturer’s instructions.}}

Long non-coding RNA LOC554202 promotes acquired gefitinib resistance in non-small cell lung cancer through upregulating miR-31 expression

^{_{Journal of Cancer    |     15 Oct 2019    |    Doi: https://doi.org/10.7150%2Fjca.35097}}

^{_{The mutation of miR-31 binding sites in 3'-UTRs of RASA1 (UCUUGCC was mutated to UGUUCGG) or FIH-1 (UCUUGCC was mutated to UGUUCGG) was performed by a Muta Direct Site-directed Mutagenesis kit (SDM-15; Beijing SBS Genetech Co, Ltd, Beijing, China).}}

Long Non-coding RNA H19 Inhibits Adipocyte Differentiation of Bone Marrow Mesenchymal Stem Cells through Epigenetic Modulation of Histone Deacetylases

^{_{Scientific Reports    |     28 June 2016    |    DOI: https://doi.org/10.1038/srep28897}}

^{_{Site-directed mutagenesis of the H19 sequences was performed using the Site-Directed Mutagenesis Kit (SBS Genetech, Beijing, China).}}