All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: MPKMB-50 (for 50T)

Cat. No.: MPKMB-200 (for 200T)

Cat. No.: MPKMB-800 (for 800T)

Description

mRNA Purification Kit with Magnetic Beads is a reagent kit used for the stable, efficient, and convenient isolation and purification of high-purity intact mRNA from total RNA. It utilizes magnetic beads coated with Oligo(dT)25, along with an optimized buffer system. The purified mRNA obtained using this kit can be directly used in various molecular biology experiments such as RT-PCR, qPCR, high-throughput sequencing, mRNA library construction, solid-phase cDNA library construction, Northern blot analysis, RACE, and mRNA vaccine research, among others.

In a typical mammalian cell, the mass and proportion of the four major macromolecules are approximately as follows: RNA, ~20 pg (1%); DNA, ~7 pg (0.3%); protein, ~500 pg (20%); polysaccharide, ~2 μg (78.7%). Messenger RNA (mRNA) accounts for approximately 4% of the total RNA mass, while ribosomal RNA (rRNA) accounts for about 80%.

The surface of the Oligo(dT)25 magnetic beads is covalently modified with a 25-mer oligo(dT) sequence. When total RNA extracted from eukaryotic cells or animal and plant tissues is mixed with the Oligo(dT)25 magnetic beads, the oligo(dT) sequence on the bead surface specifically binds to the poly(A) tail at the 3' end of mRNA through base pairing. Then, under the influence of an external magnetic field, the magnetic beads can be rapidly and efficiently separated from the corresponding solution, followed by thorough washing to remove impurities. Finally, the mRNA is eluted from the magnetic beads using an elution solution, resulting in the obtainment of high-purity intact mRNA.

Components

50T

• Oligo (dT)25 Magnetic Beads    1ml
• Solution I (Binding Buffer)    30ml
• Solution II (Wash Buffer)    25ml
• Solution III (Elution Buffer)    2ml

Advantages

• Stable extraction performance, high purity, fast speed, and convenient operation
• Extensively tested and optimized mRNA extraction system
• Isolates and purifies over 90% of mRNA from total RNA
• Complete the purification process in less than 15 minutes
• Simple steps including incubation, washing, and elution
• All operations are conducted in a single centrifuge tube for convenience

Product Information

Oligo (dT)25 magnetic beads in this kit have a particle size of approximately 200 nm and a concentration of approximately 5 mg/ml. Each milligram of magnetic beads is coupled with approximately 300-400 pmol of Oligo (dT)25, allowing purification of approximately 2-3 µg of mRNA per milligram of beads.

For routine mRNA purification, using 20 μl of magnetic bead suspension per sample, the small packaging of this kit can be used for 50 mRNA purifications, the medium packaging for 200 mRNA purifications, and the large packaging for 800 mRNA purifications.

Precautions

• Please ensure strict measures to prevent contamination with RNAases and DNAases during the operation.
• All reagents and consumables used in this product must be RNase-free and DNase-free. Exercise caution during the procedure to avoid contamination. If there is a possibility of RNase contamination in the consumables, consider soaking them overnight in 0.01% DEPC-treated water, followed by high-temperature and high-pressure sterilization, and drying. High-temperature and high-pressure sterilization is generally effective in inactivating DNases if there is a possibility of DNase contamination.
• A magnetic separation device is required for use and should be obtained separately. We recommend using our VSep™ Magnetic Separators.
• When aliquoting or working with the magnetic beads, it is recommended to vortex or invert them gently to ensure thorough mixing.
• Before magnetic separation, gently agitate the centrifuge tube to ensure even dispersion of the magnetic beads before bringing them close to the magnetic field. If the magnetic beads stick to the tube wall, gently shake the liquid in the tube to make the beads flow down after they aggregate.
• Please use the recommended amount of total RNA. If the total RNA amount is too high, it may cause bead aggregation, which can affect washing and, consequently, the purity of the extracted mRNA. In the case of bead aggregation, it is advisable to disperse the beads as much as possible during washing to improve the extraction efficiency. If bead aggregation occurs, it is recommended to reduce the total RNA amount in subsequent experiments.
• Due to the susceptibility of RNA degradation, it is recommended to use the extracted mRNA promptly for downstream experiments such as RT-PCR. If immediate use is not possible, store the mRNA at -80°C.
• This product is intended for scientific research by professionals only and should not be used for clinical diagnosis or treatment, food or drug purposes, or stored in ordinary residential areas.
• For your safety and health, please wear appropriate laboratory attire and disposable gloves while handling.

Storage

Store at 4°C for up to one year, ensuring optimal stability.

If the Oligo (dT)25 magnetic beads are not used for an extended period, long-term storage at -20°C is recommended to maintain their quality over an extended period.

Related: Animal mRNA Isolation Kit with Magnetic Beads

SBS Genetech is recognized as one of the global major leading industry players in mRNA Raw Material by third-party market researchers. For more details, please visit Top 5 mRNA Vaccine & Therapeutics Raw Material Market Companies in Global Market 2022.

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory