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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
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    • Ecosystem
    • Legal Statement
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Mitochondrial Protein Extraction Kit

Mitochondrial Protein Extraction Kit

$133.00
Mitochondrial Protein Extraction Kit is used for extracting mitochondrial proteins from mammalian tissues or cells. The principle involves first isolating intact mitochondria from animal tissues or cells, and then lysing the mitochondria with a lysis buffer containing protease and phosphatase inhibitors to obtain high-purity mitochondrial proteins. This kit can be used for subsequent studies such as Western Blot and co-immunoprecipitation.
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More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: MPEK-50 (for 50T)

 

Description

Mitochondrial Protein Extraction Kit is used for extracting mitochondrial proteins from mammalian tissues or cells. The principle involves first isolating intact mitochondria from animal tissues or cells, and then lysing the mitochondria with a lysis buffer containing protease and phosphatase inhibitors to obtain high-purity mitochondrial proteins. This kit can be used for subsequent studies such as Western Blot and co-immunoprecipitation.

 

Components

50T

  • Mitochondrial Protein Extraction Reagent A
  • Mitochondrial Protein Extraction Reagent B
  • Mitochondrial Protein Extraction Reagent C
  • 0.1M PMSF 
  • 1M DTT
 

Storage

Store at -20°C. This kit is valid for one year from the date of purchase.

 

Notes

  • Perform all steps at low temperatures.
  • Small-scale preparations are more convenient and faster than large-scale ones, making it easier to obtain intact mitochondria.
  • Disrupting cells without damaging subcellular organelles is the most crucial step in mitochondrial preparation. Compared to tissue blocks, cultured cells, especially adherent cells, are more difficult to lyse with a glass homogenizer. Therefore, use a small-capacity glass homogenizer and a well-fitting pestle to grind adherent cells. Under a light microscope, about 50% of cells should remain unbroken. Excessive grinding can damage the mitochondria, while insufficient grinding will lower the yield.
  • Calculate the correct centrifugation speed based on the centrifugal force in g. The centrifugation speed can be precisely calculated for different centrifuges. The relative centrifugal force (RCF) value (in g) depends on the rotor speed (rpm) and the radius of rotation (r, in mm) and can be expressed using the formula: RCF = 1.11 × 10⁻⁶ × r × (rpm)².
  • For Western Blot and 2D electrophoresis, mitochondrial lysates can be directly added to sample buffer.
  • PMSF must be added within 2-3 minutes before adding the lysis buffer to the sample to prevent PMSF from becoming unstable and ineffective in aqueous solution.
  • This kit is suitable for fresh tissue samples only and is less effective for previously frozen tissues.

 

 

 

 

Only for research and not intended for treatment of humans or animals

 

 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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