- All Products
- Custom Services
- Catalog Products
- Isothermal Portfolio
- Nucleic Acid Related
- Enzymes
- Innovative Systems
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: MRRE-50 (for 50T)
Cat. No.: MRRE-200 (for 200T)
Description
In recent years, due to extensive research on RNA interference and regulatory small RNAs, there has been an urgent need for a kit capable of efficiently extracting RNA of approximately 15-30 nucleotides in size (including siRNA and miRNA). However, traditional RNA extraction methods like silica gel membranes cannot effectively adsorb and recover these molecules. Phenol/guanidine extraction and ethanol precipitation also fail to effectively precipitate and recover small molecule RNAs.
This kit uses a unique lysis solution combined with β-mercaptoethanol to rapidly lyse cells and inactivate cellular RNases. It employs a robust organic extraction process to remove proteins and DNA. RNA, including small molecule RNA, is adsorbed onto a special silica-based membrane inside the centrifuge column. This is followed by a series of rapid wash-centrifugation steps using a washing solution, which further removes cellular metabolites, proteins, and other impurities. Finally, the RNA is eluted from the silica-based membrane using a low-salt elution solution.
Features
Only for research and not intended for treatment of humans or animals
Journals Using SBS Genetech Products Universities Using SBS Genetech Products
SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory