All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: LPLC-50 (for 50ml)

Cat. No.: LPLC-250 (for 250ml)

Description

After concentration and purification, the viral stock significantly enhances viral titer and purity, thus leading to more efficient cell infection. Currently, there are three commonly used concentration methods available. 

Firstly, the ultra-high-speed centrifugation method (recommended for animal experiments) involves setting the centrifugal force above 90000g and following the steps for ultra-high-speed centrifugation. 

Secondly, ultrafiltration concentration (recommended for establishing stable transfection cell lines) entails transferring the viral liquid to a 15mL ultrafiltration tube (Millipore Ultra-15; 100kD) and concentrating it to 0.2-1mL. 

The most recommended method is the third one, utilizing LentiPro Lentivirus Concentrator (5×). This concentrator allows for a simple and rapid concentration of all types of lentivirus particles. This concentrator is easy to use – just mix the 5× concentrate with lentivirus supernatant in proportion and incubate briefly. Afterward, use a regular centrifuge (at 4°C, 4000g for 25 minutes). This process yields concentrated lentivirus particles with a recovery rate of over 90%, resulting in an increase in virus titer by up to 100-fold.

Component

LentiPro Lentivirus Concentrator (5×)

Features

• User-friendly Operation: Using LentiPro Lentivirus Concentrator is extremely simple. Just mix the concentrator with lentivirus supernatant in proportion, undergo a brief incubation, followed by standard centrifugation steps, and you'll obtain concentrated lentivirus particles.
• Efficient Concentration: This concentrator is capable of rapidly and effectively concentrating all types of lentivirus particles. With a recovery rate exceeding 90%, it can elevate viral titers by up to 100-fold.
• Wide Compatibility: LentiPro Lentivirus Concentrator is suitable for various types of lentivirus particles, ensuring satisfying concentration results regardless of the particle type.

Protocol

1. Collect lentivirus supernatant and filter through a 0.45 μm membrane to remove cells and debris.

• Note: When using a filtration membrane, only use cellulose acetate or polyethersulfone (PES) membranes with low protein binding capacity. Nitrocellulose (NC) membranes should not be used.

2. Mix lentivirus supernatant with the concentrator from the kit at a 4:1 ratio (4 parts supernatant, 1 part concentrator). Incubate at 4°C for 2 hours or overnight. Initially, mix the solution every 30 minutes for a total of 3 times.

• Note: Prolonging the incubation time can enhance the recovery rate. Lentivirus particles can be stored stably at 4°C for several days.

3. Centrifuge at 4°C, 4000g for 25 minutes.

4. Carefully remove the supernatant without disturbing the tube, usually revealing a white pellet (sometimes not visible).

5. Re-suspend the viral pellet in 1/10 to 1/100 of the initial volume (original supernatant volume) of DMEM or PBS. Gently pipette and mix to re-suspend the pellet.

6. Determine the titer of the concentrated lentivirus sample, aliquot in 50µl volumes per tube, and store at -80°C.

• Note: Avoid repeated freeze-thaw cycles for lentivirus cryopreserved samples to prevent a decrease in viral titer.

Storage

The product should be stored at 4°C and has a shelf life of 6 months.

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory