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Cat. No.: HPBLSM-200 (for 200ml)

Description

Human Peripheral Blood Lymphocyte Separation Medium is a separation solution based on the principle of density gradient centrifugation. It allows for the simple, rapid, and efficient isolation of lymphocytes, monocytes, and other cells from human peripheral blood, umbilical cord blood, or bone marrow cells. It is also known as Lymphocyte Separation Medium (LSM), lymphocyte gradient separation medium, density gradient medium, PBMC separation medium, PBMC sample density separation medium, or PBMC cell separation medium. The density of this separation medium is 1.077 g/ml at 20ºC, which is nearly iso-osmotic with whole peripheral blood.

Different blood cells in human peripheral blood vary in terms of volume, morphology, and specific gravity (density). Red blood cells and granulocytes have a higher specific gravity, around 1.092, while mononuclear cells (lymphocytes and monocytes) have a specific gravity of approximately 1.075, and platelets have a specific gravity of about 1.030. Therefore, by using a separation solution with a density ranging from 1.075 to 1.090, lymphocytes and monocytes, which have lower specific gravity, float in this separation medium, while red blood cells and granulocytes, with higher specific gravity, sink. This allows for the isolation of mononuclear cells from peripheral blood cells. The most commonly used separation solution is a mixture with a density of approximately 1.070, prepared using polysucrose 400/Ficoll 400 and sodium diatrizoate.

The human peripheral blood lymphocyte separation medium we provide has undergone formulation optimization and improvement. At 20ºC, its density is approximately 1.077 g/ml, which is nearly iso-osmotic with peripheral blood. During density gradient centrifugation using this separation medium, the cells or liquid in the centrifuge tube separate into four layers from top to bottom: the plasma and low-density platelets form the first layer, suspended in the upper part of the separation medium; the mononuclear cells (PBMC, including lymphocytes and monocytes) have a slightly lower density than the separation medium and are located above the interface of the separation medium. They usually appear as a circular milky-white layer, which is the second layer. By removing the first layer, PBMC can be obtained. The third layer is a transparent separation medium layer, and the red blood cells and granulocytes, with higher density, settle at the bottom, forming the fourth layer.

Features

• This product offers a user-friendly experience with easy operation, a short processing time, and a high rate of lymphocyte separation. The entire experiment can be completed in approximately one hour by following simple pipetting and centrifugation steps. The product achieves an extraction efficiency of over 80% for lymphocyte separation. To obtain target cells of high purity, immunomagnetic beads can be used in combination. By utilizing this reagent kit for pre-separation, the required amount of magnetic beads can be reduced, resulting in cost savings.
• This product maintains a high standard of quality through stringent quality control measures. The separation solution is formulated using pharmaceutical-grade raw materials and optimized for optimal performance. The manufacturing process takes place under sterile conditions, ensuring a sterile product with endotoxin levels below 0.5 EU/ml.
• This product has a wide range of applications and is suitable for both diluted and undiluted anticoagulated blood samples. It effectively separates lymphocytes and can also obtain plasma of a certain purity. The isolated lymphocytes can be directly analyzed or utilized for cell culture purposes.