Human Cot-1 DNA Pro (500 μg)
$400.00
$500.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: HC1DP-500 (for 500 μg)
Description
Human Cot‑1 DNA Pro is derived from genomic DNA extracted from human placenta. It consists of highly repetitive sequences approximately 50–300 bp in length. These repetitive elements effectively block repetitive regions present in probes or target nucleic acids, thereby preventing nonspecific hybridization signals during nucleic acid hybridization. By masking repetitive sequences, Human Cot‑1 DNA Pro enhances hybridization specificity and improves the efficiency of target sequence capture.
Experimental results demonstrate that Human Cot‑1 DNA Pro significantly reduces false‑positive signals caused by repetitive sequences in chromosome in situ hybridization, lowers background noise, and improves the accuracy and reliability of hybridization assays. Most importantly, Human Cot‑1 DNA Pro features shorter and more uniform fragment sizes compared with conventional Human Cot‑1 DNA, resulting in superior suppression of nonspecific DNA binding.
Application
Human Cot‑1 DNA Pro is widely used in:
- Northern blotting
- Southern blotting
- In situ hybridization, including tissue ISH, somatic cell ISH, and FISH
- Liquid‑phase hybridization capture
Additionally, Human Cot‑1 DNA Pro can be used as a library screening probe for identifying clones in somatic cell hybrid libraries or flow‑sorted chromosome libraries derived from somatic cell hybrids.
Superior Blocking Performance
- Utilizes a proprietary fragmentation process to ensure that ≥50% of DNA fragments fall within the 50–300 bp range.
- Achieves a blocking efficiency of over 95%, significantly outperforming comparable products.
- Effectively blocks common repetitive sequence families such as Alu and LINE.
Rigorous Quality Control Standards
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High Batch-to-Batch Consistency
- Manufactured using standardized production processes.
- Each batch undergoes comprehensive QC testing.
- A detailed Certificate of Analysis (COA) is provided with every batch.\
Comparison with Human Cot‑1 DNA
- DNA Fragment Pattern Comparison: Human Cot‑1 DNA Pro exhibits shorter and more uniform fragment sizes compared with conventional Human Cot‑1 DNA. This tighter distribution enhances its ability to suppress nonspecific DNA binding.
- Blocking Efficiency Evaluation: Under identical input conditions, Human Cot‑1 DNA Pro demonstrates lower background intensity and a higher signal‑to‑noise ratio compared with both conventional Human Cot‑1 DNA and the no‑Cot‑1 control. With equivalent target signal strength, Human Cot‑1 DNA Pro provides superior blocking performance, making it particularly well‑suited for FISH applications.
Related:
Only for research and not intended for treatment of humans or animals
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