
Heparin Magnetic Agarose Beads
$72.00 - $1,600.00
$2,000.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: HEPMAB-2 (for 2 mL)
Cat. No.: HEPMAB-10 (for 10 mL)
Cat. No.: HEPMAB-50 (for 50 mL)
Cat. No.: HEPMAB-200 (for 200 mL)
Description
Heparin Magnetic Agarose Beads are made by covalently coupling high-quality heparin to agarose magnetic beads. Also known as heparin magnetic beads or Magrose Heparin, they can rapidly and efficiently separate biomolecules with an affinity for heparin, including antithrombin III, thrombin, clotting factors, and other plasma proteins. They can also be used to purify lipoproteins, nucleases, steroid receptors, cell growth factors, interferons, nucleic acid-binding proteins, viruses, and other biological macromolecules. Heparin agarose magnetic beads are widely used for the purification of various proteins and viruses and can achieve binding, washing, and elution of target proteins by altering the salt concentration in the buffer. This product can also be used to remove residual nucleases, including RNase and DNase.
Heparin, commonly available as heparin sodium in its sodium salt form, is a highly sulfated linear polysaccharide primarily composed of 1,4-glycosidic linkages between pyranose uronic acids (L-iduronic acid and D-glucuronic acid) and 2-amino-2-deoxyglucose (glucosamine) residues, with an average molecular weight of about 15 kDa. Heparin is mainly produced by mast cells and basophils and is found in high amounts in the liver, lungs, blood vessel walls, intestinal mucosa, and mast cells. Its interactions with heparin-binding proteins regulate many important physiological processes, such as complement cascade activity, cell differentiation, growth, migration, inflammation, and pathogen infection. The interaction between heparin and most proteins primarily depends on the high negative charge density of the sulfonic and carboxyl groups in heparin, which can form ionic bonds with the basic amino acids (arginine and lysine) in the proteins. Some protein-heparin interactions are due to hydrogen bonding between heparin domains and polar amino acids (asparagine and glutamine); for example, brain natriuretic peptide (BNP) interacts with heparin mainly through hydrogen bonds. Additionally, the flexible conformation of L-iduronic acid in heparin can form specific structural domains that bind proteins, such as unique nucleic acid-like domains that specifically bind nucleases or unique virus receptor-like domains that specifically bind viruses.
Due to the biological activity of heparin's anticoagulant properties, affinity for factor proteins, nucleic acid-like domains, and virus receptor-like domains, heparin agarose magnetic beads are extensively used in protein purification. They can specifically purify antithrombin III, thrombin, coagulation factors IX (FIX), XI (FXI), apolipoproteins, and nucleic acid-binding proteins; purify transcription factors, nucleases, DNA polymerases, and endonucleases; and are used for the separation and purification of viral vaccines and viral vectors. They are also used for purifying cell growth factors, interferons, and other biological macromolecules.
Features
- High Binding Capacity Compared to similar products, this product has a very high binding capacity, allowing rapid separation and purification of biomolecules such as antithrombin III, coagulation factors, and growth factors from complex samples. The heparin agarose magnetic beads are provided as a 10% suspension (containing 100 µl of sediment per ml of product). Each ml of heparin agarose magnetic beads sediment is coupled with 4 mg of heparin, capable of binding ≥2 mg of antithrombin III, thus enabling efficient adsorption and purification experiments.
- Strong Specificity This product can specifically bind to antithrombin III, coagulation factors, growth factors, apolipoproteins, and nucleic acid-binding proteins. The obtained products are of high purity and can be further used in Western blotting, ELISA, mass spectrometry, and other subsequent analytical tests.
Product Content
10% slurry in specific protective buffer
Average Particle Size
30~150μm
Magnetization
Superparamagnetic
Coupled Ligand
Heparin
Ligand Concentration
~4mg Heparin per ml Beads
Binding Capacity
Per ml Beads: ≥2mg antithrombin III (ATIII)
Specificity
Electrostatic effect
Application
Separation of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors, other plasma proteins, DNA-binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors, viruses, etc.
Storage Conditions
Store at -20ºC for one year. At 4ºC, the product is valid for at least one month.
Precautions
- Maintain pH between 6-8 and avoid high-speed centrifugation and drying. Do not leave the magnetic beads in a magnetic field for extended periods as this may cause aggregation.
- Before use, properly resuspend the beads by inverting the vial several times to mix the agarose beads evenly. Mix gently and avoid vigorous vortexing.
- The product contains trace amounts of preservatives. Before use, wash the beads three times with an appropriate solution such as TBS to eliminate potential interference from the preservatives.
- When performing immunoprecipitation or purification, it is recommended to include both positive and negative control groups.
- After collecting protein samples, complete the purification process as quickly as possible and keep samples at 4ºC or on ice to reduce protein degradation or denaturation. Adding a suitable protease inhibitor cocktail to the protein samples can effectively inhibit protein degradation.
- If centrifugation does not fully remove insoluble materials from the protein samples, filter the sample solution using a 0.45μm filter.
- A 0.1% non-ionic detergent (such as Triton X-100, Tween-20, or NP-40) can effectively prevent bead aggregation without affecting the beads' protein-binding efficiency.
- Agarose beads should not be reused after elution with acidic solutions or SDS-PAGE. To minimize heparin loss, whether manually or automatically operated, the low-pH elution step should not exceed 10 minutes.
- High concentrations of DTT, beta-mercaptoethanol, or guanidine hydrochloride may affect the binding of the product to ligands.
- This product is intended for scientific research use by professionals only and must not be used for clinical diagnostics or treatments, food or drug applications, and must not be stored in a regular household.
Only for research and not intended for treatment of humans or animals
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