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HEK293 Residual DNA Detection Kit (qPCR)

HEK293 Residual DNA Detection Kit (qPCR)

$480.00 - $1,440.00
$1,800.00
HEK293 Residual DNA Detection Kit (qPCR), also referred to as the Human Genomic DNA Residue Probe qPCR Kit, HEK293 Genomic DNA Residue Probe qPCR Kit, or HEK293 Host Cell Genomic DNA Residue Detection Kit, is a probe‑based quantitative PCR (qPCR) assay designed for rapid, efficient, highly sensitive, and highly specific quantification of residual genomic DNA derived from HEK293 cells, human host cells, or human tissues in various biological product samples.
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All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: HRDDK-50 (for 50T)

Cat. No.: HRDDK-200 (for 200T)

 

 

Description

HEK293 Residual DNA Detection Kit (qPCR), also referred to as the Human Genomic DNA Residue Probe qPCR Kit, HEK293 Genomic DNA Residue Probe qPCR Kit, or HEK293 Host Cell Genomic DNA Residue Detection Kit, is a probe‑based quantitative PCR (qPCR) assay designed for rapid, efficient, highly sensitive, and highly specific quantification of residual genomic DNA derived from HEK293 cells, human host cells, or human tissues in various biological product samples.

This kit is formulated with an anti‑contamination system containing high‑quality UDG enzyme and dUTP at optimized ratios, effectively eliminating false‑positive results or abnormally low Ct values caused by carryover contamination from previous PCR amplifications.

HEK293 cells (Human Embryonic Kidney 293 Cells), also known as human embryonic kidney 293 cells, exhibit high transfection efficiency, strong adaptability, and the ability to grow in adherent or suspension culture. They are widely used in cell biology research and in the expression and production of target proteins, bioactive molecules, and viral products, including recombinant proteins, antibodies, vaccines, as well as lentivirus, adenovirus, and adeno‑associated virus (AAV)–based biopharmaceuticals.

Biological products manufactured using HEK293 cells may contain trace amounts of residual HEK293 cell‑derived DNA fragments. These fragments may pose biosafety risks, and elevated levels of residual DNA have been associated with increased risks of inflammation, oncogenicity, and immune dysregulation. Regulatory agencies in many countries and regions impose strict limits on host cell DNA residues in biopharmaceutical products. Monitoring and controlling host cell DNA levels is essential for evaluating the immunogenicity risk and ensuring the safety of biological products.

This kit enables highly sensitive detection of residual DNA from HEK293 host cells, with a limit of detection in the femtogram (fg) range. Based on the quantitative PCR method used in exogenous DNA residue assays, the probe‑based qPCR workflow achieves a detection limit of 30 fg/μl and a quantitative range of 30 fg/μl to 300 pg/μl.

 

Components

  • Probe qPCR Mix (2X, UDG)
  • Primer/Probe Mix (10X)
  • Ultrapure Water
  • Dilution Buffer
  • HEK293 DNA Control (30ng/μl)
  • Low ROX (50X)
  • High ROX (50X)

 

Features

  • This kit exhibits high specificity, and genomic DNA from CHO cells or E. coli does not interfere with the detection of HEK293 DNA residues. The assay exclusively detects HEK293 host cell DNA and shows no cross‑reactivity with DNA from other species.
  • This kit demonstrates excellent stability, and varying degrees of DNA fragmentation have minimal impact on the detection of HEK293 DNA residues. HEK293 DNA Control was fragmented to different extents using micrococcal nuclease (MNase), followed by qPCR analysis with this kit. The results showed that DNA fragments across different fragmentation levels were consistently detected, indicating that fragmentation does not affect assay performance.
  • This kit demonstrates excellent repeatability. When 3 pg/μl and 30 fg/μl HEK293 DNA samples were tested in 12 replicates, the coefficient of variation (CV) for both concentrations was less than 5%.
  • This kit provides the essential components required for qPCR, including the Probe qPCR Mix (2X, UDG) and a premixed set of specific primers and probe. The premix contains high‑quality Taq DNA polymerase, UDG enzyme, PCR buffer, dNTPs, dUTP, stabilizers, and magnesium ions, covering all universal reaction components. The primers and probe are designed against human‑specific repeat sequences and have been optimized to achieve ultra‑high sensitivity for quantifying HEK293 DNA or other human genomic DNA samples. In addition, the kit includes an HEK293 DNA Control, which serves as a positive control to verify kit performance and to generate standard curves.
  • This product is available in both Low ROX and High ROX formats, ensuring broad compatibility with real‑time PCR instruments that require no ROX, Low ROX, or High ROX as a passive reference dye. ROX functions as an internal fluorescence normalization dye to correct for non‑PCR‑related signal fluctuations, thereby minimizing well‑to‑well variation. Such variation may arise from factors including pipetting errors or sample evaporation. Different qPCR instruments have different ROX requirements; therefore, users should select the appropriate Probe qPCR Mix (2X) containing High ROX, Low ROX, or no ROX based on the instrument in use. In general, Probe qPCR Mix (2X, High ROX) is also compatible with instruments that require Low ROX or no ROX. The ROX requirements for commonly used qPCR instruments are summarized in the table below.

 

Storage

Store at -20ºC for one year. Primer/Probe Mix (10X), Low ROX (50X), and High ROX (50X) should be stored protected from light and avoid repeated freeze-thaw cycles.

 

Precautions

  • It is recommended to use this product immediately after first opening, or to properly aliquot and store it at -20ºC under sterile conditions to prevent contamination by airborne E.coli, which could affect experimental results.
  • Ensure that the reagents are completely thawed and thoroughly mixed before use. Avoid generating bubbles during mixing.
  • Primer/Probe Mix (10X), Low ROX (50X), and High ROX (50X) contain fluorescent dyes. Avoid strong light exposure when storing this product or setting up the PCR reaction system to prevent fluorescence quenching.
  • Tests have shown that repeated freeze-thaw cycles up to 10 times do not significantly affect the product’s performance, but it is still recommended to avoid repeated freeze-thaw cycles as they may degrade product performance.
  • qPCR detection is highly sensitive; it is recommended to perform the detection in a standard PCR laboratory. The PCR reaction setup area should be free from potential amplification product contamination. Although this product is contamination-resistant, it is still advised not to tear open the PCR sealing film or open the PCR tube cap in the PCR reaction setup area. PCR products should be sealed and handled according to post-amplification product requirements to avoid contamination of the experimental environment with high concentrations of PCR products.
  • It is recommended to use filter tips to prepare the PCR system to minimize false positives caused by contamination.
  • It is recommended to use low-adsorption 1.5 ml centrifuge tubes for high sample recovery rates.
  • This product is for scientific research use only by professionals and is not intended for clinical diagnosis or treatment, food, or drugs, and should not be stored in ordinary residential areas.
  • For your safety and health, please wear a lab coat and disposable gloves during operation.

 

Related:

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 
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