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Cat. No.: GOD-20 (for 20KU)

Cat. No.: GOD-200 (for 200KU)

Cat. No.: GOD-35m (for 35MU)

Description

CAS: 9001-37-0

Glucose Oxidase (GOD), produced through genetic engineering of the glucose oxidase gene from Aspergillus niger in recombinant Saccharomyces cerevisiae, is a yellow lyophilized powder. It is free from animal-derived components.

In terms of functionality, GOD exhibits high specificity in converting β-D-glucose, while having low efficiency towards other monosaccharides. It demonstrates fast oxygen removal, excellent thermal stability, and does not generate precipitates or turbidity after reactions.

In the food industry, GOD serves as a green and environmentally friendly additive with no toxicity or side effects on human health. It can prevent beer aging, preserve the original flavor, and extend the shelf life. It enhances the stability of red wine and grape juice. When applied in packaged foods, it acts as a deoxygenation agent, prolonging the preservation period. It is also utilized for the preservation of milk, fruit juice, vegetables, and more.

Another significant application of GOD is in determining glucose content in samples. By utilizing its specific oxidase properties, GOD-based analyzers and test papers enable rapid, accurate, and convenient measurement of glucose content in various food products, as well as for blood glucose testing in humans. Currently, glucose oxidase finds widespread applications in the food industry, biopharmaceutical sector, and beyond.

Specification

• CAS Number: 9001-37-0
• E.C. Number: 1.1.3.4
• Source Species: Recombinant Saccharomyces cerevisiae expressing genetically modified glucose oxidase gene from Aspergillus niger
• Catalog Number: GPE008001
• Molecular Weight: 153 kD
• Specific Activity: ≥ 50 U/mg (Lyophilized Powder)
• Dilution Buffer: 20 mM Tris-Cl (pH 8.0), 2 mM MgCl₂, 20 mM NaCl
• Storage Buffer: 20 mM Tris-Cl (pH 8.0), 2 mM MgCl₂, 20 mM NaCl, 50% glycerol
• Inhibitors: p-Chloromercuribenzoate (pCMB), Heavy metal ions (Cu²⁺, Hg²⁺, Ag⁺, etc.)
• Temperature Range: 40-50℃, optimum temperature at 42℃
• pH Range: 4-7, optimum pH at 4.5
• Transport, Storage, and Stability: Lyophilized powder formulation can be transported at room temperature. After receipt, it is recommended to store in a dry environment below 4°C (or -20℃) with a shelf life of 3 years. Liquid products have a shelf life of 1 year. Opened packages should be used promptly and protected from moisture.
• Unit of Activity Definition: The enzyme activity is defined based on TOYOBO's measurement method as the amount of enzyme required to generate 1 µmol of hydrogen peroxide (or 0.5 µmol of quinoneimine dye) per minute.