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Cat. No.: FPET-50 (for 50T)
Cat. No.: FPET-200 (for 200T)
Description
This kit is designed for rapid extraction of total RNA from formalin-fixed, paraffin-embedded tissue samples. A unique lysis buffer/proteinase K quickly lyses cells to release RNA. The lysis mixture then passes through a genomic DNA clearance column, where genomic DNA is removed while RNA penetrates and filters through. After adjusting the filtered RNA with ethanol to set binding conditions, RNA selectively binds to the silica matrix membrane inside the centrifuge column under high salt conditions. Through a series of rapid wash-centrifugation steps, protein removal and wash solutions eliminate cellular metabolites, proteins, and other impurities. Finally, RNase-free water with low salt elutes the pure RNA from the silica matrix membrane. The obtained RNA is suitable for experiments such as reverse transcription PCR and fluorescent quantitative PCR.
Features
Storage
This kit can be stored at room temperature for 12 months without affecting its performance.
Only for research and not intended for treatment of humans or animals
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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory