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Cat. No.: FPET-50 (for 50T)

Cat. No.: FPET-200 (for 200T)

Description

This kit is designed for rapid extraction of total RNA from formalin-fixed, paraffin-embedded tissue samples. A unique lysis buffer/proteinase K quickly lyses cells to release RNA. The lysis mixture then passes through a genomic DNA clearance column, where genomic DNA is removed while RNA penetrates and filters through. After adjusting the filtered RNA with ethanol to set binding conditions, RNA selectively binds to the silica matrix membrane inside the centrifuge column under high salt conditions. Through a series of rapid wash-centrifugation steps, protein removal and wash solutions eliminate cellular metabolites, proteins, and other impurities. Finally, RNase-free water with low salt elutes the pure RNA from the silica matrix membrane. The obtained RNA is suitable for experiments such as reverse transcription PCR and fluorescent quantitative PCR.

Features

• Completely avoids the use of toxic reagents like phenol and chloroform, and there's no need for steps like ethanol precipitation.
• It's fast and straightforward; RNA extraction from an individual sample can generally be completed within 1 hour.
• The kit's exclusive genomic clearance column and formulation ensure the effective removal of residual genomic DNA. In most cases, the obtained RNA does not require DNase digestion and can be used directly for experiments like reverse transcription PCR and fluorescent quantitative PCR.
• Multiple column washes ensure high RNA purity, making it directly suitable for a variety of downstream experiments.

Storage

This kit can be stored at room temperature for 12 months without affecting its performance.