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Cat. No.: FDS10K-50 (for 50mg)

Cat. No.: FDS10K-250 (for 250mg)

Cat. No.: FDS10K-1k (for 1g)

Description

FITC‑labeled Dextran Sulfate Sodium, abbreviated FITC‑DSS, also known as FITC‑Dextran Sulfate Sodium Salt, FITC‑labeled Dextran Sulfate (sodium salt), Fluorescein isothiocyanate–Dextran Sulfate (sodium salt), or Fluorescein‑labeled Dextran Sulfate (sodium salt), is a widely used fluorescent tracer compound commonly applied in the establishment of colitis animal models.

Dextran Sulfate Sodium (DSS) is a sulfated derivative of dextran. It is typically produced as a white powder by reacting dextran of defined molecular weight with sulfating agents such as chlorosulfonic acid, followed by purification. The DSS‑induced model is considered the gold standard for generating inflammatory bowel disease (IBD) models, and is widely used to induce colitis in mice, rats, zebrafish, and Drosophila. Its mechanism involves disrupting gut microbiota homeostasis, impairing macrophage function, and exerting cytotoxic effects on colonic epithelial cells. During use, DSS is often conjugated with fluorescein isothiocyanate (FITC) to visualize its distribution and potential sites of action. For example, FITC‑labeled DSS enables clear microscopic observation of tissue permeability changes in the colonic epithelium shortly after DSS exposure. FITC‑labeled DSS of different molecular weights also helps researchers rapidly assess cell membrane pore size. In addition, when analyzing mucus barrier alterations in IBD patients, FITC‑labeled DSS can be used to evaluate how DSS penetrates the mucus plume.

The FITC‑labeled Dextran Sulfate Sodium product series uses FITC to label DSS of various molecular weights. The labeling process ensures that DSS remains intact without degradation, while maintaining an appropriate degree of FITC substitution along the polysaccharide chain. This provides sufficient fluorescence intensity, minimizes potential alterations to the intrinsic properties of DSS caused by the labeling reaction, and effectively reduces unwanted interactions with intracellular components.

Our FITC‑labeled Dextran Sulfate Sodium product series is supplied as a lyophilized powder, processed through precipitation, repeated ethanol washing, and fluorescence‑intensity verification to ensure the complete removal of free dye. These FITC‑labeled DSS products feature high purity, excellent quality, good stability, and a broad molecular‑weight range, allowing users to select the most suitable molecular weight according to experimental objectives.

FITC is a widely used green fluorescent probe that emits green fluorescence upon excitation, enabling detection of FITC‑labeled DSS using fluorescence microscopy or fluorescence plate readers. The absorption (excitation) and emission maxima of FITC are listed below:

The basic properties of this FITC‑labeled Dextran Sulfate Sodium series are as follows:

The labeling efficiency corresponds to approximately 0.1–2 FITC molecules per 100 glucose units, equivalent to 0.001–0.02 moles of FITC per mole of glucose.

This FITC‑labeled Dextran Sulfate Sodium series appears yellow to orange and exhibits high solubility in water, saline solutions, and many organic solvents such as DMSO, formamide, ethylene glycol, glycerol, and hexamethylphosphoramide. It is insoluble in lower aliphatic alcohols, acetone, chloroform, and dimethylformamide.

Storage

Store at -20ºC, protected from light. Valid for at least one year.

Precautions

• Fluorescent substances are prone to quenching, so please protect them from light.
• This product series is in dry powder form and easily absorbs moisture from the air. Please avoid leaving it exposed and ensure it is kept dry.
• This product series is intended for scientific research use by professionals only. It is not for clinical diagnosis or treatment, not for use in food or drugs, and should not be stored in residential areas.
• For your safety and health, please wear a lab coat and disposable gloves when handling.

FITC-labeled Dextran:

• FITC-labeled Dextran (MW 4000)
• FITC-labeled Dextran (MW 10000)
• FITC-labeled Dextran (MW 40000)
• FITC-labeled Dextran (MW 70000)
• FITC-labeled Dextran (MW 150000)

FITC-labeled Dextran Sulfate Sodium:

• FITC-labeled Dextran Sulfate Sodium (MW5,000)
• FITC-labeled Dextran Sulfate Sodium (MW10,000)
• FITC-labeled Dextran Sulfate Sodium (MW40,000)
• FITC-labeled Dextran Sulfate Sodium (MW500,000)

Only for research and not intended for treatment of humans or animals

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