FII Magnetic RNA-Protein Pull-Down Kit (Microorganism)
$690.00 - $1,520.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: FMPDKM-12 (for 12 reactions)
Cat. No.: FMPDKM-24 (for 24 reactions)
Cat. No.: FMPDKM-40 (for 40 reactions)
Description
FII Magnetic RNA-Protein Pull-Down Kit utilizes a 16-nt RNA tag, "F2," which binds to the target RNA. Through the strong affinity of the specific ligand attached to the F2 tag and fixed on magnetic beads, the target RNA is efficiently retrieved along with associated proteins. Subsequently, specific proteins can be detected using Western Blot, or unknown proteins can be identified using mass spectrometry (LC-MS/MS).
Applications
RNA-binding proteins (RBPs) are a powerful and widely distributed class of regulatory factors, comprising about 5%-10% of all cellular proteins. While a few RNAs function independently as ribozymes, most RNAs exert their functions by forming RNA-protein complexes through binding with proteins. On one hand, RBPs are involved in RNA regulation, and on the other hand, RNA can regulate RBPs. The FII Magnetic RNA-Protein Pull-Down Kit can be applied to several research areas, including:
- Regulation of RNA activity, transport, and localization;
- RNA stability, translation, and degradation;
- RNA synthesis and alternative splicing;
- RNA modifications;
- Activity, localization, or interactions of RBPs with other proteins.
Advantages
- The F2 tag is a short RNA sequence that can bind and label various types of RNA.
- The F2 tag is only 16 nt long, which has minimal impact on the structure or function of the target RNA.
- It can be used to label target RNA both in vivo and in vitro.
- The F2 tag has a strong affinity for its specific ligand, allowing for efficient retrieval of F2-RNA and its associated proteins.
RNA Pull-Down Kit Product Comparison Table
Brief Usage Procedure
- Obtain target RNA-F2 through in vitro transcription or in vivo expression.
- Capture the target RNA-F2 using the specific ligand on magnetic beads.
- Incubate with the protein sample to retrieve proteins bound to the target RNA.
Only for research and not intended for treatment of humans or animals
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