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Cat. No.: FTRE-50 (for 50T)

Cat. No.: FTRE-200 (for 200T)

Description

The unique lysis solution combined with β-mercaptoethanol swiftly breaks down cells and inactivates cellular RNA enzymes. After adjusting the binding conditions with ethanol, RNA selectively adsorbs to the silica matrix membrane inside the centrifuge column under high salt conditions. Through a series of rapid wash-centrifugation steps, the protein removal liquid and wash solution eliminate cellular metabolites, proteins, and other impurities. Finally, RNase-free H2O with low salt concentration elutes the pure RNA from the silica matrix membrane.

Features

• The silica matrix membrane inside the centrifugal adsorption column is exclusively sourced from a world-renowned company, ensuring minimal adsorption differences between columns and good repeatability. This overcomes the inconsistency in membrane quality found in common kits.
• There is no need to use toxic reagents like phenol and chloroform, nor is there a requirement for steps like ethanol precipitation.
• The process is rapid and straightforward; the operation for a single sample can generally be completed within 30 minutes.
• Multiple column washes ensure high purity. The typical OD260/OD280 ratio reaches 1.9 to 2.0, with virtually no DNA residues remaining. The purified product is suitable for RT-PCR, Northern-blot, and various other experiments.

Storage

This kit can be stored at room temperature for 6 months without affecting its performance.