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Cat. No.: EXN8T-500 (for 500U)

Cat. No.: EXN8T-2k (for 2KU)

Cat. No.: EXN8T-10k (for 10KU)

Description

Exonuclease VIII, Truncated, also known as truncated Exonuclease VIII, originates from Escherichia coli. Exonuclease VIII, truncated, has 5’ to 3’ DNA exonuclease activity, specifically cleaving linear double-stranded DNA. It exhibits similar enzymatic activity on 5’ ends that are blunt or sticky, and on 5’ ends with either phosphate or hydroxyl groups. However, Exonuclease VIII, truncated, cannot cleave circular DNA. Experimental results show that Exonuclease VIII, truncated, can digest single-stranded DNA with 5’ ends that are either phosphorylated or hydroxylated from the 5’ to 3’ direction, but its activity on single-stranded DNA with 5’ phosphorylated ends is significantly higher than on those with 5’ hydroxylated ends.

Application

Digests linear double-stranded DNA while maintaining circular double-stranded DNA.

Source

Expressed and purified from Escherichia coli.

Unit Definition

One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble deoxyribonucleotide from double-stranded DNA in a total reaction volume of 50 µl in 30 minutes at 37ºC in 1X Exonuclease VIII, truncated Buffer with 0.15 mM sonicated duplex [3H] DNA.

Purity

Free from other types of exonucleases, endonucleases, RNases, and phosphatases, except for Exonuclease VIII, truncated.

Inactivation or Inhibition

Heating at 70ºC for 15 minutes will inactivate Exonuclease VIII, truncated.

Storage

Store at -20ºC, effective for at least two years.

Precautions

• When using this product, it should be kept in an ice box or ice bath. After use, it should be immediately stored at -20ºC.
• This product is for scientific research use only by professionals. It is not intended for clinical diagnosis or treatment, nor for use in food or drugs, and should not be stored in a residential area.
• For your safety and health, please wear a lab coat and disposable gloves while handling.