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Cat. No.: ENDOH-25k (for 25KU)

Description

Endoglycosidase H, abbreviated as Endo H, is an endoglycosidase enzyme derived from Streptomyces plicatus and recombinantly expressed in E. coli. It specifically cleaves high-mannose and some hybrid glycoproteins by breaking the β-1,4-glycosidic bond, leaving a glycoprotein structure with a single N-acetylglucosamine residue. The cleaved proteins retain their biological activity, allowing for subsequent functional studies without interference. Endo H is primarily used for removing N-linked glycans from proteins.

Source

Derived from Streptomyces plicatus and recombinantly expressed in E. coli.

Enzyme Activity Definition

One unit of enzyme activity is defined as the amount of enzyme required to remove more than 95% of carbohydrates from 10 μg of denatured RNase B in 1 hour at 37°C.

Purity and Concentration

Purity >95% as determined by SDS-PAGE; endogenous nucleic acid content <1 pg/μL (as detected by qPCR); 500 U/μL.

Inactivation or Inhibition

Inactivated by heating at 75°C for 10 minutes.

Enzyme Storage Buffer

20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, 50% glycerol, pH 7.5.

10× Denaturing Buffer

5% SDS, 400 mM DTT.

10× Native Buffer

400 mM sodium acetate, pH 6.0.

Storage

Shipped on wet ice; store at -20°C, with a shelf life of 12 months.

Only for research and not intended for treatment of humans or animals

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