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Cat. No.: DPMB-50 (for 50T)

Cat. No.: DPMB-200 (for 200T)

Description

DNA Purification Kit with Magnetic Beads is a type of magnetic bead-based nucleic acid purification medium, designed for the stable, efficient, and convenient extraction and purification of DNA from PCR products or various DNA reaction systems.

This kit is suitable for the removal of impurities such as primers, enzymes, mineral oil, glycerol, salts, etc., following PCR reactions. It is also equally applicable for the purification of DNA after reactions such as restriction enzyme digestion, ligation, phosphorylation, fill-in or blunt-end reactions, random priming, and more. The purified DNA obtained can be directly used for subsequent processes such as restriction digestion, ligation, bacterial transformation, sequencing, PCR, hybridization, and other applications.

The DNA in the sample specifically binds to the magnetic beads, and under the influence of an external magnetic field (such as a magnetic separation rack), the magnetic beads can be rapidly and efficiently separated from the corresponding solution. After thorough washing to remove impurities, the DNA is finally eluted from the magnetic beads using elution buffer, resulting in highly purified DNA samples.

Components

• Magnetic Beads
• Solution I (Binding Buffer)
• Solution II (Wash Buffer)
• Solution III (Elution Buffer)

Features

• This kit offers several advantages, including stable and effective results, high purity, flexible and convenient operation, and the ability to purify DNA fragments within a broad size range. The amount of magnetic beads can be adjusted flexibly according to the specific requirements, typically allowing purification to be completed within 15 minutes. It is suitable for the purification of DNA fragments larger than 100 base pairs, and it can also achieve satisfactory results for purifying DNA fragments as large as 10 kilobases. It can effectively remove primers with a length of up to 30 nucleotides. Typically, the recovery rate is above 90%. This kit is also suitable for the concentration of low-concentration samples.
• Currently, the primary method for DNA purification is column purification kits. This method typically requires repetitive centrifugation or specialized filtration devices, and the fiber cutting that occurs during column purification is not particularly conducive to the recovery of large DNA fragments. In contrast, the magnetic bead method operates under mild conditions, eliminating the need for cumbersome centrifugation or filtration steps and replacing them with a simple magnetic adsorption process, ensuring a quick and convenient operation.
• Compared to traditional purification methods, this kit does not involve the use of toxic reagents like phenol/chloroform during the purification process.

Storage

Store at room temperature, effective for one year. When Magnetic Beads are not in use for an extended period, they can be stored at 4°C, which allows for even longer storage.

Note

• You will need to provide anhydrous ethanol and a magnetic separation apparatus. 
• Before the first use, add the specified amount of anhydrous ethanol to each bottle of Solution II (Wash Buffer), mix well, and label the bottles accordingly.
• Magnetic bead suspension may settle when left undisturbed. Before use, ensure thorough mixing by vortexing or inverting several times.
• Before magnetic separation, gently shake the centrifuge tube to disperse the magnetic beads fully before bringing them close to the magnetic field. If bead aggregation occurs, gently agitate the liquid inside the tube to allow the adhered beads to flow down.
• At lower temperatures, Solution I (Binding Buffer) may precipitate. Prior to use, check for any precipitation. If present, heat the solution in a 37°C water bath to dissolve the precipitate, mix well, and then use.
• This product is suitable for manual extraction and can also be used with workstations or nucleic acid extraction instruments.
• Deionized water can be used instead of the elution buffer, but the pH of the deionized water should not be lower than 6.5. If it is lower, adjust it to 7.5-8.5 with a low-concentration NaOH solution.
• Solution I (Binding Buffer) may have some irritant effects on the human body. Please handle it with care and take appropriate precautions.
• This product is for research use by professionals only and should not be used for clinical diagnosis or treatment. It should not be used in food or drugs and should not be stored in residential homes.
• For your safety and health, wear lab attire and disposable gloves while handling the product.

Only for research and not intended for treatment of humans or animals

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