All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: DFTTH-500 (for 500U)

Cat. No.: DFTTH-2500 (for 2500U)

Cat. No.: DFTTH-10k (for 10KU)

Cat. No.: DFTTH-25k (for 25KU)

Description

Tth DNA Polymerase is a thermostable DNA polymerase derived from thermophilic bacteria and recombinantly expressed in Escherichia coli, with a molecular weight of 94 kDa. This enzyme has been specially engineered to possess strong amplification capacity and rapid extension speed. It can amplify DNA fragments up to 3–5 kb, with an extension rate of 1–2 kb/min at 72 °C.

The enzyme exhibits 5'→3' polymerase activity and weak 5'→3' exonuclease activity, but lacks 3'→5' exonuclease activity. Amplification products carry a 3'-dA overhang.

In addition, Tth DNA Polymerase displays reverse transcriptase activity in the presence of Mn²⁺. Leveraging this property, the enzyme enables reverse transcription and PCR to be performed in a single tube (one-step RT-PCR).

Our DNA-Free PAGE Taq DNA Polymerase is devoid of genomic and plasmid DNA from the production strain. This makes this polymerase particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like PCR and primer extension.

Features

• Completely free from bacterial DNA.
• This specially engineered enzyme delivers robust amplification capability and fast extension speed. It can efficiently amplify DNA fragments of 3–5 kb at an extension rate of 2 kb/min.
• Excellent thermostability: half-life exceeds 40 min at 95 °C.
• Tolerates dUTP and dITP.
• PCR products contain a 3'-dA overhang, suitable for direct T/A cloning.

Applications

• Conventional PCR amplification
• Colony PCR
• Generation of PCR products with 3'-dA overhangs for TA cloning
• Conventional PCR following reverse transcription

Unit Definition

The amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into acid-insoluble material within 30 minutes at 72°C, using salmon sperm DNA as the template/primer, is defined as one activity unit (U).

Storage

Related: 

• DNA-Free HS-Taq DNA Polymerase (for hot-start PCR)
• DNA-Free HiFi Taq DNA Polymerase (for high-fidelity PCR)
• DNA-Free HS HiFi Taq DNA Polymerase (for hot-start & high-fidelity PCR)
• DNA-Free Taq-D DNA Polymerase (for direct PCR)
• DNA-Free HS Taq-D DNA Polymerase (for hot-start & direct PCR)
• DNA-Free LATaq DNA Polymerase (for long DNA PCR)
• DNA-Free HS LATaq DNA Polymerase (for hot-start & long DNA PCR)
• DNA-Free PAGE Taq DNA Polymerase (for PAGE)
• DNA-Free Pfu DNA Polymerase
• DNA-Free HS Pfu DNA Polymerase
• DNA-Free KOD DNA Polymerase
• DNA-Free KOD Plus DNA Polymerase

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory