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Cat. No.: DFTMAE3-500 (for 500U)

Cat. No.: DFTMAE3-2500 (for 2500U)

The lyophilized Tma Endonuclease III is also available by inquiry, which can be transported at room temperature.

Description

Tma Endonuclease III is derived from a thermophilic bacterium and is a homologous protein to E. coli Endonuclease III, displaying excellent thermal stability. It possesses both N-glycosylase activity and AP-lyase activity. The N-glycosylase activity can release oxidatively damaged pyrimidine bases occurring on double-stranded DNA, generating an apurinic/apyrimidinic (AP) site. Subsequently, the AP-lyase activity cleaves at this site. Tma Endonuclease III recognizes DNA damage, including abasic sites on DNA, 5,6-dihydroxythymine, and thymine glycol. Our Tma Endonuclease III is recombinantly expressed and prepared through multi-step purification of the recombinant protein.

Our DNA-Free Tma Endonuclease III, on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes DNA-Free Tma Endonuclease III particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like PCR and primer extension.

Activity Definition

One unit of activity is defined as the amount of enzyme required to cleave 1 pmole of a 60-mer oligonucleotide double-stranded DNA containing a single AP site in 1 hour at 65°C in a reaction buffer system of 1× Tma Endonuclease III.

Activity Assay Conditions

1× AM Buffer E: 20 mM Tris-HCl (pH 8.8), 10 mM KCl, 10 mM (NH4)2SO4, 2 mM MgSO4, 0.1% Triton X-100, incubation at 65°C.

Concentration

10 U/μl

Applications

• Thermally stable enzyme capable of performing DNA repair reactions at high temperatures.
• Single-cell gel electrophoresis (comet assay).
• DNA damage repair.

Storage

Store at -20°C for up to 2 years, avoiding repeated freeze-thaw cycles.

Only for research and not intended for treatment of humans or animals

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