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Cat. No.: DFT4DL-10k (for 10KU)

Cat. No.: DFT4DL-50k (for 50KU)

Description

DNA-Free T4 DNA Ligase, also known as T4 DNA ligase, catalyzes the formation of phosphodiester bonds between the 5'-P and 3'-OH termini of double-stranded DNA or RNA with sticky or blunt ends. Additionally, T4 DNA ligase can repair single-strand nicks in double-stranded DNA, double-stranded RNA, or DNA/RNA hybrids but is inactive on single-stranded nucleic acids. T4 DNA ligase requires ATP as a cofactor for the ligation reaction.

Our DNA-Free enzymes are all recombinant proteins and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.

Unit Definition

One unit is defined as the amount of enzyme required to ligate 50% of HindIII-digested λ DNA fragments [5´ end concentration of 0.12 μM (300 μg/ml), total DNA amount of 6 μg] in a 20 μl reaction within 30 minutes at 16℃ in 1× T4 DNA Ligase reaction buffer.

Features

• Completely free from bacterial DNA.
• Sticky end ligation completed in just 1 minute.
• Heat inactivation: 65℃ for 10 minutes.

Applications

• Restriction enzyme cloning.
• Efficient ligation of sticky and blunt ends.
• T/A cloning.
• Construction of high-abundance clone libraries.
• RNA and DNA ligation.
• Repair of single-strand nicks in double-stranded DNA, RNA, or DNA/RNA hybrids.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory