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Cat. No.: DFAPS-300 (for 300U)

Cat. No.: DFAPS-1500 (for 1500U)

Description

DNA-Free Alkaline Phosphatase (Shewanella) (ShAP) catalyzes the hydrolysis of phosphate monoesters but does not hydrolyze phosphate diesters or triesters. It can be irreversibly inactivated by heat treatment at 70℃ for 15 minutes.

Our DNA-Free enzymes are all recombinant proteins and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.

Unit Definition

One unit of activity is defined as the amount of enzyme required to hydrolyze 1 μmol of p-nitrophenyl phosphate to p-nitrophenol per minute at 37℃ and pH 9.8 under 1× Alkaline Phosphatase (Shewanella) reaction buffer conditions.

Features and Applications

• Completely free from bacterial DNA.
• Colorimetric reactions of alkaline phosphatase, including enzyme-linked immunodetection.
• Pre-treatment of PCR products for sequencing when combined with Exonuclease I. Note: Not recommended for dephosphorylation of vectors used in ligation reactions. For such needs, please select AE1802A/B, AE1803A/B, AE1807A/B based on your specific requirements.
• Removal of 5’ phosphate groups from DNA molecules.
• Active between 20-60℃.
• Irreversibly inactivated by heating at 70℃ for 15 minutes.

Storage

Store at -20℃ for up to 2 years. Avoid repeated freeze-thaw cycles.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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