DirectTaq™ HF DNA Polymerase (Blood-Resistant) is a highly efficient heat-resistant DNA polymerase modified by genetic engineering and suitable for direct PCR detection of EDTA, heparin, or sodium citrate anticoagulant samples or dry blood spot samples.
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Cat. No.: DTHFB-200 (for 200T)
Cat. No.: DTHFB-1k (for 1000T)
DirectTaq™ HF DNA Polymerase (Blood-Resistant) can perform PCR on whole blood samples without extracting DNA. The polymerase shows strong resistance to various PCR inhibitors such as heme in humans, mouse and other whole blood. It does not need DNA extraction and purification for human or mouse blood samples or dry blood spots (such as dry blood spots on whatman903 or FTA cards) freshly collected or stored at 4°C or frozen at low temperature with EDTA, heparin or sodium citrate, and can be directly used for PCR amplification of target DNA.
Easy operation: The polymerase can perform PCR on whole blood samples without extracting DNA.
Less sample required: Usually only about 1 μl anticoagulated blood is required for a 20 μl reaction system.
High compatibility: The polymerase is suitable for direct PCR detection of EDTA, heparin, or sodium citrate anticoagulant samples or dry blood spot samples.
Strong tolerance: The tolerance volume percentage of the polymerase to human whole blood treated with heparin, sodium citrate, and EDTA can reach 50%, 20%, and 40%.
Good amplification: The polymerase can easily amplify the target template up to 5kb or longer.
High fidelity: The fidelity of the polymerase is about 5 times higher than Pfu DNA Polymerase.
Phenol-chloroform extraction can inactivate DirectTaq™ HF DNA Polymerase (Blood-Resistant).