DirectTaq™ HF DNA Mastermix (Blood-Resistant) is a high fidelity PCR mastermix specially used for the direct PCR of blood samples. After PCR, the sample can be directly loaded for electrophoresis without adding a loading buffer. DirectTaq™ HF DNA Mastermix (Blood-Resistant) contains DirectTaq™ HF DNA Polymerase (Blood-Resistant), which is a highly efficient heat-resistant DNA polymerase modified by genetic engineering and suitable for direct PCR detection of EDTA, heparin or sodium citrate anticoagulant samples or dry blood spot samples.
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: DTHFMB-100 (for 100T)
Cat. No.: DTHFMB-500 (for 500T)
DirectTaq™ HF DNA Polymerase (Blood-Resistant) can perform PCR on whole blood samples without extracting DNA. The polymerase shows strong resistance to various PCR inhibitors such as heme in humans, mouse, and other whole blood. It does not need DNA extraction and purification for human or mouse blood samples or dry blood spots (such as dry blood spots on whatman903 or FTA cards) freshly collected or stored at 4°C or frozen at low temperature with EDTA, heparin or sodium citrate, and can be directly used for PCR amplification of target DNA.
Ready-to-use: Only primers, samples, and water are required for the reaction.
Easy operation: The polymerase can perform PCR on whole blood samples without extracting DNA. After PCR, the sample can be directly loaded for electrophoresis without adding a loading buffer.
Less sample required: Usually only about 1 μl anticoagulated blood is required for a 20 μl reaction system.
High compatibility: The polymerase is suitable for direct PCR detection of EDTA, heparin, or sodium citrate anticoagulant samples or dry blood spot samples.
Strong tolerance: The tolerance volume percentage of the polymerase to human whole blood treated with heparin, sodium citrate, and EDTA can reach 50%, 20%, and 40%.
Good amplification: The polymerase can easily amplify the target template up to 5kb or longer.
High fidelity: The fidelity of the polymerase is about 5 times higher than Pfu DNA Polymerase.
Phenol-chloroform extraction can inactivate DirectTaq™ HF DNA Polymerase (Blood-Resistant).
DNA synthesis is a technology that links deoxynucleic acids (adenine, thymine, cytosine, and guanine) together to form DNA. As the cornerstone of modern molecular biology, DNA synthesis plays a pivotal role in the field of synthetic biology. In addition to standard oligos synthesis, we also provide scientific research services such as long oligos synthesis, phosphorothioate oligos (S-Oligo) synthesis, modified oligos synthesis, fluorescent oligos synthesis, and real-time quantitative PCR probes to meet your different needs. Besides, we have been providing high-quality DNA synthesis products (phosphoramidites, controlled pore glass, molecular sieves, etc), and the products have been successfully applied in various fields of molecular biology.
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A peptide library is a new technique for studying the structure-function relationship for a protein. A peptide library contains a great number of peptides that have a systematic combination of amino acids. The peptide library provides a powerful tool for drug design, protein-protein interactions, and other biochemical as well as pharmaceutical applications. It also has wide applications in drug screening, target validation, epitope mapping, and vaccine development.
Gene synthesis is a technology that synthesizes genes by artificial methods, which is one of the means of gene acquisition. Compared with the acquisition of genes from existing organisms, gene synthesis does not need templates and is therefore not limited by the source of genes. We use unique gene synthesis design software, which includes a full set of tools to design ideal structural units, thus enabling rapid and efficient gene construction and synthesis in a single reaction. Please do not be limited by restriction sites and polylinkers, we will synthesize the various gene sequences you need.
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Ribonucleic acid (RNA), as a key material for genetic information transmission and cell regulation, has been extensively studied in molecular biology. Like DNA, RNA is assembled in the form of nucleotide chains; but unlike DNA, RNA exists in nature in the form of single-stranded folds rather than paired double strands. We provide RNasin (RNase inhibitor) and M-MLV reverse transcriptase to provide a complete raw material solution for RNA research. miAnalysis™ series are designed for microRNA quantitative research. And VirusMag™ series is designed for the isolation of viral RNA/DNA or bacterial DNA.
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RNA silencing technology has become a powerful tool to study gene function. The success of any RNA experiment depends on high-quality siRNA and effective transfection reagent. With chemical modification, our chemically modified siRNA has much higher stability than the common siRNA. The chemical modification not only enhances the life span of siRNA in serum and cell culture but also enhances its activity in vitro. As for transfection reagent, our ready-to-use siRNA transfection reagent, Sirnafectamine, can be used for a wide range of cell lines, with minimal cytotoxicity and the best cell state after transfection. As Sirnafectamine will protect RNA during the whole process, a very low concentration of siRNA can produce high gene silencing efficiency.
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CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria and archaea. The gene-editing technology based on this system has a wide variety of applications. Here, we provide various Cas nucleases, synthetic sgRNAs, and T7 Endonuclease I of high quality to improve the accuracy and efficiency of your experiments. We also offer the Cas13a Nuclease (Lyophilized) and Cas12a Nuclease (Lyophilized) that can be transported at room temperature, saving the high cost of dry ice transportation.
Gene manipulation is a process that uses biotechnology to manipulate genes directly to generate new DNA and has been widely used in research, medicine, industrial biotechnology, and agriculture. Our Premium™ Master Assembly Mix and Topo Cloning kits (pBM23, pBM16A, pBM16K) provide efficient solutions for these types of demands.