DirectTaq™ HF DNA Mastermix (Blood-Resistant) is a high fidelity PCR mastermix specially used for the direct PCR of blood samples. After PCR, the sample can be directly loaded for electrophoresis without adding a loading buffer. DirectTaq™ HF DNA Mastermix (Blood-Resistant) contains DirectTaq™ HF DNA Polymerase (Blood-Resistant), which is a highly efficient heat-resistant DNA polymerase modified by genetic engineering and suitable for direct PCR detection of EDTA, heparin or sodium citrate anticoagulant samples or dry blood spot samples.
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: DTHFMB-100 (for 100T)
Cat. No.: DTHFMB-500 (for 500T)
DirectTaq™ HF DNA Polymerase (Blood-Resistant) can perform PCR on whole blood samples without extracting DNA. The polymerase shows strong resistance to various PCR inhibitors such as heme in humans, mouse, and other whole blood. It does not need DNA extraction and purification for human or mouse blood samples or dry blood spots (such as dry blood spots on whatman903 or FTA cards) freshly collected or stored at 4°C or frozen at low temperature with EDTA, heparin or sodium citrate, and can be directly used for PCR amplification of target DNA.
Ready-to-use: Only primers, samples, and water are required for the reaction.
Easy operation: The polymerase can perform PCR on whole blood samples without extracting DNA. After PCR, the sample can be directly loaded for electrophoresis without adding a loading buffer.
Less sample required: Usually only about 1 μl anticoagulated blood is required for a 20 μl reaction system.
High compatibility: The polymerase is suitable for direct PCR detection of EDTA, heparin, or sodium citrate anticoagulant samples or dry blood spot samples.
Strong tolerance: The tolerance volume percentage of the polymerase to human whole blood treated with heparin, sodium citrate, and EDTA can reach 50%, 20%, and 40%.
Good amplification: The polymerase can easily amplify the target template up to 5kb or longer.
High fidelity: The fidelity of the polymerase is about 5 times higher than Pfu DNA Polymerase.
Phenol-chloroform extraction can inactivate DirectTaq™ HF DNA Polymerase (Blood-Resistant).
DNA synthesis is a technology that links deoxynucleic acids (adenine, thymine, cytosine, and guanine) together to form DNA. As the cornerstone of modern molecular biology, DNA synthesis plays a pivotal role in the field of synthetic biology. In addition to standard oligos synthesis, we also provide scientific research services such as long oligos synthesis, phosphorothioate oligos (S-Oligo) synthesis, modified oligos synthesis, fluorescent oligos synthesis, and real-time quantitative PCR probes to meet your different needs.
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