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Cat. No.: AVEGP-50 (for 50T)

Cat. No.: AVEGP-100 (for 100T)

Description

In normal cells, phosphatidylserine (PS) is distributed exclusively on the inner side of the lipid bilayer of the cell membrane. However, in the early stages of apoptosis, phosphatidylserine (PS) flips from the inner to the outer side of the lipid bilayer. Annexin V is a 35-36 kDa Ca²⁺-dependent phospholipid-binding protein with a high affinity for phosphatidylserine. Therefore, it can bind to the cell membrane of early apoptotic cells through the exposed phosphatidylserine on the outer side of the cell. Annexin V is thus used as a sensitive marker for detecting early apoptosis. When Annexin V is labeled with fluorescein (EGFP, FITC), it serves as a fluorescent probe. Using fluorescence microscopy or flow cytometry, one can detect apoptosis. Compared to the green fluorescence signal of FITC, the green fluorescence signal of EGFP is stronger, more resistant to photobleaching, and more stable. Therefore, this kit uses EGFP as the fluorescent labeling probe.

Propidium iodide (PI) is a nucleic acid dye that cannot pass through intact cell membranes. However, it can penetrate the cell membranes of late apoptotic and dead cells, staining their nuclei red. By using Annexin V and PI together, one can distinguish cells at different stages of apoptosis.

This kit is suitable for detecting apoptosis in cultured cells (not recommended for tissue samples).

Components

• AnnexinV-EGFP
• Propidium Iodide
• Binding Buffer