This terminal transferase (TdT) has a molecular weight of 58.3 kDa and no 5' and 3' exonuclease activities. The addition of Co2+ to the reaction can improve the tailing efficiency. The enzyme is widely used in DNA 3' end addition homopolymer, DNA 3' end labeling using modified bases (such as ddNTP, DIGdUTP), TdT-mediated dUTP nick end labeling technology (in situ detection of apoptosis) and other tests. The enzyme is tested free of DNA endonuclease and exonuclease contamination and RNase.
- Oligonucleotide or DNA 3' hydroxyl end labeling
- DNA tailing
- Synthesis of oligomeric strands of the same deoxynucleotide
The amount of enzyme required to catalyze the addition of 1 nmol dNTP to the 3' hydroxyl end of a polynucleotide within 60 min at 37℃ is defined as an active unit.
1 x TdT Buffer
100 mM KCl, 30 mM Tris-acera, 0.05% (v/v) Triton X-100 (pH 7.5 at 25℃)
Stored at -20℃ for 3 years.
75℃ for 20 min.
- The activity of Terminal Transferase can be lost by appropriate amount of EDTA.
- Metal ion chelators, higher concentrations of ammonium ions, chloride ions, iodine ions and phosphate ions all have inhibitory effects on Terminal Transferase activity.