for Superior Biology Services
for Superior Biology Services
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: 3173DP-500 (for 500U)
Cat. No.: 3173DP-5KU (for 5KU)
3173 DNA Polymerase is a thermostable DNA polymerase derived from a virus. Through point mutation modification, its 3 '- 5' exonuclease activity is deleted (exo form) to obtain better amplification activity. This enzyme not only has DNA-dependent polymerase activity but also has RNA-dependent reverse transcription activity, so it can be used for single-tube RT-PCR amplification. The enzyme has strong chain replacement activity and can be used for the isothermal amplification of DNA or RNA. In the LAMP experiment using RNA as a template, a single enzyme system reaction can be realized. For RNA templates with complex secondary structures, a good amplification effect can be obtained by heating at 92°C for 3s, while Bst pol will lose amplification activity after heating at this temperature.
The half-life of 3173 DNA polymerase at 95°C is 20 min, so the initial pre-denaturation time of PCR is usually 1~2 min. This enzyme is matched with two kinds of Buffer: 10 × 3173 Isothermal Buffer and 5 × Best PCR Buffer。 10 × 3173 Isothermal Buffer is used for isothermal amplification, and its reaction temperature cannot exceed 70°C. 5 × Best PCR Buffer is used for routine PCR amplification, and the pre-denaturation time at 94°C cannot exceed 5min.
3173 DNA Polymerase (5 U/μl): 100 μl
10×3173 Isothermal Buffer: 1 ml
5×Best PCR Buffer: 1 ml
One unit is defined as the amount of enzyme required to catalyze the incorporation reaction of 25nmol dNTP into acid-insoluble substances within 30 minutes at 65°C.
Strong extension ability and reverse transcription activity
Strong strand displacement activity, applicable to LAMP detection
Extremely strong heat resistance and amplification activity for complex templates
Strand displacement gene synthesis
DNA LAMP and RT-LAMP
It has reverse transcriptional activity between 60-70°C and can be used for single-tube RT-PCR
The minimum shelf life is 3 years at -20°C.
SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory
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