RNA-Related - SBS Genetech - for Superior Biology Services since 2000

mRNA Decapping Enzyme

mRNA Decapping Enzyme is a decapping enzyme that catalyzes the cleavage and removal of the 7‑Methylguanosine Cap (m7G) structure at the 5' end of mRNA, generating a 5' monophosphate end and 7‑Methylguanosine 5'‑Diphosphate (m7GDP). This enzyme can decap mRNAs of various lengths and exhibits equivalent removal efficiency toward Cap‑0 and Cap‑1 cap structures. It can also convert the 5' triphosphate end of mRNA to a 5' monophosphate end, albeit with relatively low conversion efficiency. RNA with a 5' monophosphate modification is applicable to a variety of downstream experiments, including 5' RNA Ligase‑mediated Rapid Amplification of cDNA Ends (5' RLM‑RACE), RNA‑seq, and 5'→3' exonuclease digestion.