Cas Nuclease - SBS Genetech - for Superior Biology Services since 2000

Enhanced PfAgo

PfAgo is an Argonaute protein derived from the hyperthermophilic archaeon Pyrococcus furiosus, exhibiting programmable endonuclease activity. Guided by a 5'-phosphorylated single-stranded DNA (gDNA), it specifically recognizes and cleaves complementary target DNA sequences. The guide DNA (gDNA) typically consists of a 16–18 nt, 5'-phosphorylated single-stranded oligonucleotide. The cleavage site is precisely located between the 10th and 11th nucleotides from the 5' end of the complementary guide sequence.