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Thermostable dsAP Nuclease

Thermostable dsAP Nuclease is a heat-stable endonuclease derived from thermophilic bacteria. It specifically recognizes abasic (AP) sites in double-stranded DNA and cleaves the first phosphodiester bond at the 5' end of the AP site, generating a 3' hydroxyl and a 5' deoxyribose phosphate (dRP) terminus.

RRE1 (RNA Rectriction Enzyme 1)

DNA restriction enzymes have been widely used in molecular biology for nearly fifty years. However, RNA restriction enzymes have remained largely unexplored. Existing RNA analysis methods are complex, costly, and unstable, often failing to meet the demands of modern molecular biology. RRE1 functions similarly to EcoToxN1 and belongs to the Type III toxin-antitoxin family. It specifically recognizes and cleaves the pentamer sequence (GAA↓AU) in single-stranded RNA. Unlike conventional RNA cleavage methods, RRE1 does not require guide RNA or DNA pairing, yet it maintains high specificity in target sequence cleavage. Moreover, it remains effective across a broad temperature range, making it an ideal RNA restriction enzyme for RNA studies and biosynthesis applications.

9N2 DNA Ligase

9N2 DNA Ligase catalyzes the formation of phosphodiester bonds, joining the 5´-phosphate and 3´-hydroxyl termini of two adjacent oligonucleotide strands hybridized to the same complementary target DNA strand.

T7 ARCA in vitro mRNA Synthesis Kit

Efficient translation of most eukaryotic mRNAs requires a 7-methylguanosine cap at the 5’ end and a Poly(A) tail at the 3’ end. The T7 ARCA in vitro mRNA Synthesis Kit enables the rapid synthesis of fully capped and tailed mRNA in vitro.

T4 Polynucleotide Kinase Kit

T4 Polynucleotide Kinase Kit, also known as T4 PNK Kit, is based on the kinase activity of T4 polynucleotide kinase (T4 PNK). This kit can be used to catalyze the transfer of phosphate groups from ATP to the 5’ hydroxyl groups of single-stranded or double-stranded DNA, RNA, oligonucleotides, or single nucleotides with a 3’ phosphate group, achieving 5’ end phosphorylation. Additionally, based on the phosphatase activity of T4 PNK, it can catalyze the dephosphorylation of 3’ phosphates in single-stranded or double-stranded DNA, RNA, and oligonucleotides.

T4 Polynucleotide Kinase (3' phosphatase minus)

T4 Polynucleotide Kinase (3' phosphatase minus), abbreviated as T4 PNK (3' phosphatase minus), is a type of polynucleotide 5'-hydroxyl kinase. It catalyzes the transfer of the γ-phosphate group of ATP to the 5'-hydroxyl of single-stranded or double-stranded DNA, RNA, oligonucleotides, or nucleotides with a 3'-phosphate group. Other NTPs can also undergo the same reaction: 5'-OH + NTP → 5'-P + NDP. Compared to T4 Polynucleotide Kinase, T4 Polynucleotide Kinase (3' phosphatase minus) lacks the 3' phosphatase activity, meaning it cannot catalyze the dephosphorylation of the 3' end of phosphorylated polynucleotides: 3'-P → 3'-OH + Pi (optimal pH is around 5.9).

T4 Polynucleotide Kinase

T4 Polynucleotide Kinase, abbreviated as T4 PNK, is a polynucleotide 5'-hydroxyl kinase that catalyzes the transfer of the γ-phosphate group of ATP to the 5'-hydroxyl of single-stranded or double-stranded DNA, RNA, oligonucleotides, or nucleotides with a 3'-phosphate group. The same reaction can occur with other NTPs: 5'-OH + NTP → 5'-P + NDP.

Extreme Thermostable T4 Polynucleotide Kinase (ET T4 PNK)

Extreme Thermostable T4 Polynucleotide Kinase (ET T4 PNK) is our proprietary, innovative enzyme—a thermostable homolog of T4 PNK. It retains the same function as T4 PNK, catalyzing the transfer of the γ-phosphate group from ATP to the 5’-hydroxyl end of oligonucleotides, including double-stranded or single-stranded DNA and RNA. This enzyme exhibits exceptional heat stability, maintaining full activity even after 30 minutes at 95°C. It operates within a temperature range of 50–95°C, though its activity significantly decreases below 65°C. Within 75–95°C, it maintains consistent enzymatic activity. These unique properties enable efficient processing of nucleic acid substrates with highly structured and complex configurations, as well as experimental conditions requiring extreme temperatures. ET T4 PNK possesses 5’-kinase activity but lacks 3’-kinase and phosphatase activity.

One-step mRNA Capping Kit

One-step mRNA Capping Kit is a specialized reagent kit designed to efficiently add a Cap-1 structure to mRNA in a single-step reaction. It contains Faustovirus Capping Enzyme (FCE), mRNA Cap 2'-O-Methyltransferase (mRNA 2'-O-MTase), GTP, S-adenosylmethionine (SAM), and the necessary reaction buffer, ensuring streamlined and effective capping.

mRNA Cap 2'- O-Methyltransferase

mRNA Cap 2'-O-Methyltransferase (mRNA 2'-O-MTase) is an mRNA capping enzyme that converts the Cap-0 structure into a Cap-1 structure. Utilizing S-adenosylmethionine (SAM) as a methyl donor, it transfers a methyl group to the 2'-O position of the first nucleotide adjacent to the Cap-0 structure (m7GpppNp) at the 5' end of the mRNA, resulting in the formation of Cap-1-modified mRNA (m7GpppNmp). This modification enhances mRNA stability and translation efficiency, improving expression levels after microinjection and transfection. Additionally, Cap-1 helps mRNA evade certain innate immune responses in cells.

Faustovirus Capping Enzyme (FCE)

Faustovirus Capping Enzyme (FCE) is a single-subunit mRNA capping enzyme derived from the Faustovirus S17 strain. FCE possesses the three enzymatic activities required for adding a Cap-0 structure to mRNA: Triphosphatase (TPase), Guanylyltransferase (GTase), and (Guanine-N7)-Methyltransferase (N7 MTase). It catalyzes the addition of an N7-methylguanosine cap structure (m7GpppNp) to the 5' end of eukaryotic mRNA at the triphosphate and diphosphate positions, generating mRNA with a Cap-0 structure.

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GoodView™ Nucleic Acid Stain (1 ml)

GoodView™ is a safer nucleic acid stain, an alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 268 nm and the other at 294 nm. In addition, it has one visible excitation at 491 nm. The Fluorescence emission of GoodView bound to DNA is centered at 530 nm.<br><br>Our GoodView™ Nucleic Acid Stain is included in New Products, Science Journal, January 11, 2019.

GoodView™ Ultra Nucleic Acid Stain

GoodView™ Ultra Nucleic Acid Stain is a next-generation nucleic acid stain that offers comprehensive compatibility with GoodView™ Red and GoodView™ Green, making it suitable for UV imaging systems, laser imaging instruments, and blue-light-based detection systems. It allows direct visualization of stained bands in PAGE gels without requiring UV or blue-light excitation, enabling effortless observation. Designed with safety in mind, its unique oil-based macromolecular structure (molecular weight &gt;1000) prevents penetration into living cells, significantly reducing cytotoxicity risks. At standard gel staining concentrations, it exhibits no mutagenic effects, providing a safer alternative to ethidium bromide (EB) for electrophoresis applications.

GoodView™ Red Nucleic Acid Stain

GoodView™ Red Nucleic Acid Stain is a new generation fluorescent nucleic acid dye, engineered for enhanced performance in electrophoresis applications. This oil-based macromolecular stain (molecular weight &gt;1000) exhibits no mutagenicity at standard gel staining concentrations, ensuring safe and reliable use. Through an innovative Fluorescence Resonance Energy Transfer (FRET) design, GoodView™ Red eliminates DNA band smearing and tailing issues commonly observed with similar products when staining large DNA fragments. This results in crisp band clarity, improved migration efficiency, precise quantification, uniform staining, exceptional sensitivity, and outstanding stability.

GoodView™ Green Nucleic Acid Stain

GoodView™ Green Nucleic Acid Stain is a non-volatile, oil-based macromolecule that does not easily sublimate or penetrate intact cell membranes, reducing potential cytotoxicity. At typical gel staining concentrations, no mutagenic effects have been observed. This stain offers enhanced safety, high sensitivity, and is an ideal nucleic acid dye for electrophoresis applications across various fragment sizes. It is fully compatible with standard gel imaging systems and visible light-excited gel observation devices, making it suitable for UV gel imaging systems and blue-light-excited gel observation instruments.

PrimeIAmp™ RT-LAMP-CRISPR RNA Fluorescence Detection Kit (One-Step Method)

PrimeIAmp™ RT-LAMP-CRISPR RNA Fluorescence Detection Kit (One-Step Method) utilizes CRISPR/Cas12b technology to achieve highly sensitive and specific pathogen detection in a single step. The system works through an integrated mechanism: first, the sgRNA-guided Cas12b complex binds target sequences and activates trans-cleavage activity to cut surrounding single-stranded DNA; simultaneously, the incorporated reverse transcriptase and PrimeIAmp™ Bst DNA polymerase enable rapid RNA amplification. This synergistic combination of CRISPR targeting and isothermal amplification technologies delivers exceptional performance for one-step pathogen detection with superior sensitivity and specificity.

PrimeIAmp™ LAMP-CRISPR DNA Fluorescence Detection Kit (One-Step Method)

PrimeIAmp™ LAMP-CRISPR DNA Fluorescence Detection Kit (One-Step Method) combines CRISPR/Cas12b technology with PrimeIAmp™ Bst DNA polymerase to enable rapid, one-step pathogen detection through a dual mechanism: first, the sgRNA-guided Cas12b system specifically binds target sequences and becomes activated to perform trans-cleavage of surrounding single-stranded DNA; second, simultaneous isothermal amplification by PrimeIAmp™ Bst DNA polymerase significantly enhances detection signals. This innovative integration achieves exceptional sensitivity and specificity that surpasses conventional detection methods, providing a powerful tool for accurate pathogen identification in a single streamlined workflow.

PrimeIAmp™ RT-LAMP Isothermal Amplification Kit (RNase H2 Probe Method)

PrimeIAmp™ RT-LAMP Isothermal Amplification Kit (RNase H2 Probe Method) combines ​PrimeIAmp™ Bst DNA polymerase with a ​custom-developed, heat-resistant reverse transcriptase for rapid RNA amplification. Integrated with ​thermostable endonuclease-specific probes, this system enables highly specific detection while demonstrating exceptional stability. Compared to conventional dye-based or probe-based methods, it delivers superior specificity and more reliable results.

PrimeIAmp™ LAMP Isothermal Amplification Kit (RNase H2 Probe Method)

PrimeIAmp™ LAMP Isothermal Amplification Kit (RNase H2 Probe Method) utilizes ​PrimeIAmp™ Bst DNA polymerase for rapid DNA amplification, combined with ​heat-resistant endonuclease-specific probes for highly specific detection. This system demonstrates superior stability and offers significantly enhanced specificity compared to traditional dye-based or probe-based methods.