Description

Third-generation sequencing technologies, represented by PacBio and Oxford Nanopore, with their characteristic long read lengths, have found extensive application in plant and animal genome research. Whole-genome sequencing of samples using third-generation sequencing technologies allows for the alignment of obtained 10 kb to 20 kb long reads with species reference genomes. This enables precise identification of genetic variations between the sample and the reference genome or between samples, such as structural variations (SV) and copy number variations (CNV). Detection of these large-segment sequence variations is beyond the capability of second-generation resequencing. By utilizing the identified SVs and CNVs, large-scale population-level genetic analyses can be conducted, and the genetic relationships between these variations, gene functions, and species trait variations can be examined. Third-generation resequencing represents a novel strategy for uncovering genetic variation information in species.

Analysis Content

• Quality control and statistics of sequencing data
• Alignment with reference genome
• SV detection and annotation
• Differential SV analysis across multiple samples
• Annotation and functional enrichment analysis of differential SVs across multiple samples
• Whole-genome structural variation CIRCOS plot

ONT Resequencing Sample Submission Requirements

DNA Sample

Tissue Sample