Description

During the construction of NGS libraries, whether through sample fragmentation, probe capture, or multiplex PCR to obtain DNA fragments, it's essential to add adapter sequences to each sample via ligation or PCR amplification. These adapter sequences contain primer sample tags (Index/barcode). Once added, each sample is assigned a unique "ID tag," allowing data analysts to accurately identify each sample in the vast sea of data, even after mixing.

Strict control of cross-contamination rates is crucial to ensure that each fragment receives its specific tag. This prevents mismatches due to cross-contamination, thus ensuring accurate sequencing results.

NGS Adapters

NGS Adapters Synthesis

• Purification Method: 

  • PAGE+ Purification: Each primer is purified using an independent system and disposable consumables, which minimizes the risk of mixing with other primers and effectively reduces the cross-contamination rate to as low as 0.01%.

  • HPLC+ Purification: Before purifying each primer, chromatography columns undergo multiple special washing procedures to thoroughly remove residues. A reasonable interval is maintained between different primers to differentiate purification processes, minimizing the risk of mixing with other primers and effectively reducing the cross-contamination rate to below approximately 0.05%.

• Base Range: 15-70 nt

• Cycle and Price: Please Inquire

• Shipment Content:

  • Single Tube or 96-Well Plate

  • Dry Powder or Solution

  • COA Document

Advantages

• Cross-contamination rate as low as 0.01%

• Stable modifications

• Extremely low base error rate

• Purity guaranteed

With over 20 years of leading experience in oligo synthesis, SBS Genetech is recognized as one of the global major leading industry players in Oligonucleotide Synthesis by third-party market researchers. For more details, please visit Oligonucleotide Synthesis Services Market landscape, Top Competitor Analysis, Revenue, Sales With Forecast Data from 2022 to 2028.