Description

Mouse CRISPR Knockout Pooled Library (Dual Plasmid System A) is designed for whole-genome knockout and screening in mice, targeting a total of 20,611 genes. The library comprises 67,405 knockout vectors, with each gene targeted by six gRNA knockout vectors. Additionally, there are 1,000 control vectors (including 1,000 targeting non-genic sequences). This library utilizes the lentiGuide-Puro backbone, a dual-plasmid vector system that only expresses gRNA, with the Cas9 gene on a separate vector, requiring paired usage.

• Species: Mouse

• Library Type: Knockout Library

• Plasmid System: Dual Plasmid System

• Virus Packaging System: Third Generation Virus Packaging System

• Target Genes: 20,611

• Number of gRNAs: 67,405

• Non-target Control sgRNAs: 1,000

• Tag: Puro

Featured Citations

Interested in seeing published research using our gRNAs?

DDR1 promotes breast tumor growth by suppressing antitumor immunity

_{Oncology Reports    |    27 September 2019    |    DOI: https://doi.org/10.3892/or.2019.7338}

_{Oligonucleotides with BsmB1 restriction sites for guide RNAs were synthesized by SBS Genetech Co., Ltd., then phosphorylated using T4 kinase (31). The phosphorylated oligonucleotides were cloned into LentiCRISPR v2 (Plasmid no. 52961; Addgene) and the sequences of the cloned plasmids that were extracted from numerous selected colonies were confirmed by SBS Genetech Co., Ltd.}

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory