Description

FAM Phosphoramidite is a widely used fluorescent labeling reagent designed for the synthesis of oligonucleotides. It incorporates a fluorescein (FAM) dye into DNA or RNA sequences, enabling sensitive detection in a variety of molecular biology applications.

This phosphoramidite is based on fluorescein, which exhibits strong green fluorescence with an excitation maximum around 495 nm and an emission maximum near 520 nm. It is supplied as a stable, high-purity compound suitable for automated DNA/RNA synthesis. The reagent is typically introduced at the 5′ end of an oligonucleotide, though it can also be incorporated internally depending on the synthesis strategy.

Specifications

• CAS Number: 204697-37-0
• Molecular Formula: C₄₆H₅₈N₃O₁₀P
• Molecular Weight: 843.95
• Appearance: Off-white solid
• HPLC: ≥97%
• ³¹P NMR: ≥97%

• 1H NMR

  : Conform

Key Features

• Provides bright, stable green fluorescence for sensitive detection

• Compatible with standard phosphoramidite chemistry in automated oligonucleotide synthesis

• Suitable for 5′-end or internal labeling of DNA and RNA

• High purity and stability for reliable performance

Applications 

FAM-labeled oligonucleotides are widely used in:

• Real-time PCR (qPCR) probes for quantitative nucleic acid detection

• Fluorescence in situ hybridization (FISH) for gene and RNA localization

• DNA sequencing and fragment analysis

• Molecular diagnostics and biomarker detection

• Nucleic acid tracking and imaging studies

Storage

FAM Phosphoramidite should be stored at –20 °C in a dry, light-protected environment. It is soluble in acetonitrile and other organic solvents commonly used in oligonucleotide synthesis. Proper protective equipment should be used when handling the reagent.

SBS Genetech offers a comprehensive range of phosphoramidites on the market. It is recognized as one of the global major leading industry players in Phosphoramidite by third-party market researchers. For more details, please visit Phosphoramidite - Global Forecast 2024-2030.

Only for research and not intended for treatment of humans or animals

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