Description

In vitro transcription (IVT) mRNA synthesis is a crucial step in the mRNA preparation process, with the plasmid template used for transcription, transcription and modification processes, and quality control being critically important. Our team of experts, with extensive experience in custom RNA synthesis for research and preclinical applications, offers comprehensive services including gene synthesis, plasmid preparation, plasmid linearization, in vitro mRNA synthesis and purification, and mRNA quality control. We can produce mRNA of various lengths and functionalities using in vitro transcription and offer optimization for different downstream applications, such as template optimization, codon optimization in the coding region, nucleotide modifications, and post-transcriptional modifications. In vitro transcribed mRNA can be used in research fields such as cell transfection, embryo injection, in vitro translation, mRNA vaccines, and drug development.

Advantages

• One-Stop Service
  Comprehensive service from DNA template synthesis to in vitro RNA transcription.

• Vector Optimization
  The IVT vector backbone used is optimized to achieve high in vitro transcription efficiency.

• Codon Optimization
  Codon optimization tailored to specific species to enhance mRNA translation efficiency.

• Nucleotide Modification
  Modified nucleotides can be introduced, such as pseudo-UTP and 5-methyl-CTP, to enhance mRNA translation efficiency and immune evasion properties.

• Customization to Meet Needs
  Customization options include 5' cap types (Uncapped, Cap0, Cap1) and 3' PolyA tail (No Tail, 120A Tail), which can be selected or customized to improve mRNA stability and translation efficiency.

• Comprehensive Quality Control
  Tailored quality control solutions with QC projects customized according to requirements.

Service Content

Deliverables

• Plasmid sequencing results
• Qualified mRNA samples
• Quality control and project report