Description

Circular RNA (circRNA) is an RNA molecule that forms a circular structure through typical 5’-3’ phosphodiester bonds. CircRNA is a naturally occurring single-stranded circular RNA found in organisms, often generated during processes such as intron reverse splicing (trans-splicing). Research on circular RNA can be conducted using plasmid or viral expression methods, which can then produce circRNA through processes such as reverse splicing. However, the auxiliary processes involved in generating circRNA via plasmids or viruses, such as reverse splicing, may potentially interfere with research results. Direct transfection synthesis of circRNA allows for a more direct study of circRNA's biological functions. Recent studies have shown that circRNA technology has significant advantages in vaccine development: compared to linear RNA, circRNA is resistant to degradation mediated by nucleases, has a longer half-life, and higher stability. Additionally, circRNA can avoid triggering TLR/RIG-I-mediated immune responses, reducing the likelihood of adverse reactions. Furthermore, circRNA does not require expensive modified nucleotides, potentially greatly reducing the cost of mRNA vaccines.

We have independently developed an efficient circRNA synthesis kit with high circularization efficiency and ease of operation, allowing for the convenient generation of circular RNA. Customers only need to provide the sequence, and we can offer a one-stop service from RNA synthesis to circularization. We also accept linear RNA products provided by customers and offer subsequent circularization services.

We provide not only synthesis services for circular small RNA but also a one-stop synthesis service for long-chain circular RNA (including circular coding RNA, also known as circular mRNA) from sequence to sample. In addition, linear mRNA obtained through transcription can be used for cell transfection to achieve good expression. Additionally, we provide industrial-grade circular mRNA synthesis services for vaccine development. We welcome inquiries and collaborations!

Advantages

• Technological Innovation: We utilize our proprietary RNA circularization reagent kit, achieving a circularization efficiency of over 95%.
• Comprehensive Service: We offer a one-stop service from RNA synthesis to circularization, and we also accept linear RNA products provided by customers for subsequent circularization processing.
• Short Turnaround Time: With advanced laboratory equipment and robust technical support, we strive to deliver the required products to our customers in the shortest possible time.

Deliverables

• Circular RNA
• Electrophoresis identification chart of circular RNA
• Certificate of Analysis (COA) for RNA synthesis (if applicable)