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Cat. No.: UXMM-100 (for 100U)

Cat. No.: UXMM-1k (for 100U)

Description

UltraX High-Fidelity DNA Polymerase combines the performance of Q5 and Q5U, making it a versatile all-in-one enzyme. Derived from high-fidelity DNA polymerase, it incorporates enhanced extension structures, endowing it with ultra-fidelity (comparable to Q5), long-fragment amplification capability, high yield, dUTP incorporation capability, and amplification of templates containing uracil. This enzyme facilitates easy amplification of 8kb genomic DNA and 20kb λDNA. It exhibits a extension rate of 4kb/min. The enzyme demonstrates 5’-3’ polymerase activity, strong 3’-5’ exonuclease activity, and produces blunt-end products.

Many high-fidelity polymerases, classified as type B DNA polymerases, halt replication upon encountering uracil bases in the DNA template. UltraX eliminates the uracil-binding domain, allowing it to read and amplify templates containing uracil, a feature denoted by the unique "U" characteristic. It has two significant applications: (1) for ultra-fidelity amplification of heavily modified, deaminated, or damaged DNA; (2) in ultra-fidelity amplification, when used with dUTP and thermosensitive UDG, to prevent contamination in PCR systems. Additionally, it is commonly employed in USER cloning.

Features

• Ultra-fidelity Amplification: Comparable fidelity to Q5 DNA polymerase, making it the optimal enzyme for vector construction, site-directed mutagenesis, NGS template amplification, and gene synthesis.
• Recognition of dUTP Substrate: Capable of reading and amplifying templates containing uracil, characterized by the "U" feature.
• Contamination-resistant Amplification: Can use dUTP to completely replace dTTP in PCR amplification, establishing contamination-resistant high-fidelity amplification.
• Fast Amplification: Exhibits amplification capability of 4kb/min.
• Long-fragment Amplification: Suitable for simple templates such as plasmids, λDNA (>20 kb), and complex genome templates (>8 kb).

Storage

Store at -20°C, effective for two years.

Related:

• UltraX High-Fidelity DNA Polymerase
• HotStart UltraX High-Fidelity DNA Polymerase
• UltraX High-Fidelity PCR Master Mix

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

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