Tth Thermostable RNase HII
$150.00 - $600.00
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Cat. No.: TTRHII-250 (for 250U)
Cat. No.: TTRHIII-1250 (for 1250U)
Tth Thermostable RNase HII is an endoribonuclease that specifically cleaves the phosphodiester bonds at the 5' end of RNA bases in a DNA double-strand, resulting in a nick and producing a 5' phosphate and 3' hydroxyl end. RNase HII also cleaves the RNA portion along the Okazaki fragment at multiple sites (i.e., RNase H activity). This enzyme does not hydrolyze pure DNA strands or single-stranded RNA. Our RNase HII is recombinantly expressed and purified through multiple steps.
The enzyme quantity required to cleave 100 pmol of double-stranded DNA substrate containing a single ribonucleotide at 55°C in 1× AM Buffer E within 30 minutes.
Activity Assay Conditions
AM Buffer E, incubation at 55°C.
- Heat-resistant enzyme with activity in the range of 40-75°C.
- Possesses RNase H activity simultaneously.
- Cleaving double-stranded DNA containing ribonucleotides.
- Generating double-strand breaks at sites of inserted ribonucleotides when used in conjunction with T7 Endo I.
- Degradation of the RNA portion of Okazaki fragments.
Store at -20°C.
- RNase H
- Thermostable RNase H
- Tth Thermostable RNase H
- Tth Thermostable RNase HII
- Extreme Thermostable RNase HII
- RNase HIII
Only for research and not intended for treatment of humans or animals
SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory