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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
  • Contact
  • …  
    • Home
    • Product 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Natural Compounds
      • Synthetic Biology
      • Enzymes
    • POCT Solution 
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Freeze-Drying System
      • Lateral Flow System
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Tth Thermostable RNase HII

Tth Thermostable RNase HII

$150.00 - $600.00
Tth Thermostable RNase HII is an endoribonuclease that specifically cleaves the phosphodiester bonds at the 5' end of RNA bases in a DNA double-strand, resulting in a nick and producing a 5' phosphate and 3' hydroxyl end. RNase HII also cleaves the RNA portion along the Okazaki fragment at multiple sites (i.e., RNase H activity). This enzyme does not hydrolyze pure DNA strands or single-stranded RNA. Our RNase HII is recombinantly expressed and purified through multiple steps.
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All products have special prices for bulk purchase, please contact for more details if required.

 

Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants

Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems. 

Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.

 

Cat. No.: TTRHII-250 (for 250U)

Cat. No.: TTRHIII-1250 (for 1250U)

 

 

Description

Tth Thermostable RNase HII is an endoribonuclease that specifically cleaves the phosphodiester bonds at the 5' end of RNA bases in a DNA double-strand, resulting in a nick and producing a 5' phosphate and 3' hydroxyl end. RNase HII also cleaves the RNA portion along the Okazaki fragment at multiple sites (i.e., RNase H activity). This enzyme does not hydrolyze pure DNA strands or single-stranded RNA. Our RNase HII is recombinantly expressed and purified through multiple steps.

 

Activity Definition

The enzyme quantity required to cleave 100 pmol of double-stranded DNA substrate containing a single ribonucleotide at 55°C in 1× AM Buffer E within 30 minutes.

 

Activity Assay Conditions

AM Buffer E, incubation at 55°C.

 

Features

  • Heat-resistant enzyme with activity in the range of 40-75°C.
  • Possesses RNase H activity simultaneously.
 

Applications

  • Cleaving double-stranded DNA containing ribonucleotides.
  • Generating double-strand breaks at sites of inserted ribonucleotides when used in conjunction with T7 Endo I.
  • Degradation of the RNA portion of Okazaki fragments.

 

Storage

Store at -20°C.

 

Instruction: Protocol

 

Related:

  • RNase H
  • Thermostable RNase H
  • Tth Thermostable RNase H
  • Tth Thermostable RNase HII
  • Extreme Thermostable RNase HII
  • RNase HIII

 

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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