Thermostable RNase H, derived from the extremely heat-resistant bacterium Thermus thermophilus, can specifically degrade RNA phosphodiester bonds hybridized to DNA strands at higher temperatures, while the DNA strands remain intact, thus degrading the RNA strands in the RNA/DNA hybrid strands.
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Cat. No.: TRNH-500 (for 500U)
Cat. No.: TRNH-5k (for 5,000U)
Compared with E.coli RNase H, the thermostable RNase H has higher activity above 65°C. Due to its extremely high thermal stability, thermostable Rnase H is an ideal choice for molecular biology applications requiring high-temperature reaction conditions. This protein is obtained by recombinant expression and purification of Escherichia coli.
A unit refers to the amount of enzyme required to hydrolyze 1 nmol of nucleic acid substrates from 40 pmol of 25 BP length RNA-DNA hybridization chains in a 50μl reaction system at 37°C within 20 min.
Stored at -20°C for 3 years.
10 x Rnase H Buffer
750 mM KCl, 500 mM Tris-HCl (pH 8.3), 30 mM MgCl2, 100 mM DTT.