$290.00 - $1,170.00
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Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants
Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems.
Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.
Cat. No.: RHIII-200 (for 200U)
Cat. No.: RHIII-1k (for 1000U)
RNase HIII is an endoribonuclease that cleaves the phosphodiester bonds at the 5' end of RNA bases in a DNA double-strand, similar to RNase HII. This cleavage results in a nick, generating a 5' phosphate and 3' hydroxyl end. RNase HII, however, cleaves the RNA portion along the Okazaki fragment at multiple sites (i.e., RNase H activity). The enzyme does not hydrolyze pure DNA strands or single-stranded RNA. RNase HIII prefers Mn2+ ions, while RNase HII prefers Mg2+ ions. Our RNase HIII is recombinantly expressed and purified through multiple steps.
The enzyme quantity required to cleave 100 pmol of double-stranded DNA substrate containing a single ribonucleotide at 37°C in 1× AM Buffer E within 30 minutes.
Activity Assay Conditions
AM Buffer E, incubation at 37°C.
- Prefers Mn2+ ions.
- Possesses RNase H activity simultaneously.
- Cleaving double-stranded DNA containing ribonucleotides.
- Generating double-strand breaks at sites of inserted ribonucleotides when used in conjunction with T7 Endo I.
- Degradation of the RNA portion of Okazaki fragments.
Store at -20°C.
- RNase H
- Thermostable RNase H
- Tth Thermostable RNase H
- Tth Thermostable RNase HII
- Extreme Thermostable RNase HII
- RNase HIII
Only for research and not intended for treatment of humans or animals
SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory