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Cat. No.: RPPH-200 (for 200U)

Cat. No.: RPPH-1k (for 1000U)

Description

RNA 5’ Pyrophosphohydrolase (RppH), also known as RNA 5’ pyrophosphate hydrolase, is a recombinant enzyme expressed and purified from Escherichia coli. It is used to catalyze the hydrolysis of the 5’ triphosphate of RNA (ppp-RNA), removing inorganic pyrophosphate (PPi) to produce 5’ monophosphate RNA (p-RNA). RppH protein can also cleave diadenosine pentaphosphate (Ap5A) into ADP and ATP. RppH activity requires Mg²⁺, and the catalysis reaction is as follows:

ppp-RNA → p-RNA + PPi

This product can be used for 5’ end RNA analysis, the removal of 5’ triphosphate RNA with XRN-1, and the construction of sequencing libraries after removing pyrophosphate from 5’ triphosphate small RNA.

RppH, also known as NudH/YgdP, is part of the Nudix hydrolase superfamily. In bacteria, it influences bacterial virulence, stress response, and DNA repair by controlling the degradation of hundreds of mRNAs. In E. coli, 5’ monophosphate mRNA produced by RppH catalysis can be degraded by RNase E. In vitro, 5’ monophosphate RNA produced by RppH can be hydrolyzed into single ribonucleotides by 5’-phosphate-dependent exonuclease (XRN-1).

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Source

Expressed in Escherichia coli, with the gene encoding RppH from E. coli.

Molecular Weight

Approximately 19.7 kDa.

Activity Definition

One unit is the amount of enzyme that converts 1 µg of 300 mer RNA transcript into XRN-1 digestible RNA in 30 minutes at 37ºC.

Purity

Free from DNA endonucleases and exonucleases, and RNAse.

Precautions

• During use, it is recommended to keep the product in an ice box or on an ice bath. After use, it should be immediately stored at -20°C.
• This product is intended for scientific research purposes by professionals only. It is not intended for clinical diagnosis or treatment, and should not be used for food or drug purposes. It should not be stored in a regular household setting.
• For your safety and health, please wear lab attire and disposable gloves when handling the product.

Storage

The minimum shelf life is 2 year at -20°C.

Related: 5’-Phosphate Dependent Exonuclease (XRN-1)