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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
  • Contact
  • …  
    • Home
    • Product 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Natural Compounds
      • Synthetic Biology
      • Enzymes
    • POCT Solution 
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Freeze-Drying System
      • Lateral Flow System
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Beijing SBS Genetech Co.,Ltd.
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PrimeIAmp™ HotStart Bst P SYBR Green MasterMix

PrimeIAmp™ HotStart Bst P SYBR Green MasterMix

$900.00 - $7,200.00
$9,000.00
This product is a special reagent for LAMP fluorescence detection. 2.5× PrimeIAmp™ HotStart Bst P SYBR Green MasterMix contains Helicase, dNTP, Mg2+, SYBR Green, reaction buffer salt, lyophilized excipient, and stabilizer. Bst P DNA/RNA Polymerase is a separate component. This product can not only be used as a routine test reagent but also be directly lyophilized for researchers with lyophilization experience, without adding any other auxiliary materials.
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All products have special prices for bulk purchase, please contact us for more details if required.

 

Cat. No.: ISGPMM-200 (for 200T)

Cat. No.: ISGPMM-2k (for 2000T)

 

Description

This product is a special reagent for LAMP fluorescence detection. 2.5× PrimeIAmp™ HotStart Bst P SYBR Green MasterMix contains Helicase, dNTP, Mg2+, SYBR Green, reaction buffer salt, lyophilized excipient, and stabilizer. Bst P DNA/RNA Polymerase is a separate component. This product can not only be used as a routine test reagent but also be directly lyophilized for researchers with lyophilization experience, without adding any other auxiliary materials.

Bst P DNA/RNA System has the following performance:

  1. The whole Bst P DNA/RNA System includes hot start Aptamer, which ensures that the enzyme activity blocking efficiency is >95% at <30°C, and the enzyme activity is completely released within 1 min at >60°C. This characteristic facilitates the establishment of the reaction system at room temperature and greatly reduces the non-specific amplification at low temperatures.
  2. The reaction temperature is further raised to 70°C, which greatly reduces the formation of primer dimer, improves the amplification specificity, and makes the nucleic acid release of crude samples more sufficient.
  3. The whole portfolio contains Helicase, so Premium LAMP amplification (pLAMP) is allowed without using F3/B3 primers. At the same time, Helicase has the function of assisting in strand unwinding, which further reduces the concentration of FIP/BIP primers. This will further reduce non-specific amplification and greatly improve amplification homogeneity.

 

Comparison

 

 

Storage Conditions

For long-term storage, please keep it below -20°C (valid for 12 months). The repeated freezing and thawing of the product for 10 times will not affect the performance, but repeated freezing and thawing should be avoided.

Due to the high concentration of sugar components in the products, the enzyme stored at -20°C may have crystals when it is melted. At this time, 2.5× PrimeIAmp™ HotStart Bst P Basic MasterMix can be completely melted at 37°C. However, if Bst P DNA/RNA Polymerase is melted at 30°C, a high temperature may lead to the reduction of hot start performance. Once melted, it is recommended to store the product at 2-8°C. Under this condition, the product can be stored stably for 6 months.

 

Instruction: Protocol

Related: Bst P DNA/RNA Polymerase

 

Customized Lyophilized Microbead Service

Looking for a personalized solution for your research needs? 

Look no further than our customized lyophilized microbead services! We offer lyophilized microbeads with customized primers and reaction volumes of 20-100 μl per bead, ensuring that your experiments are optimized to your exact specifications. Don't settle for a one-size-fits-all approach - Contact us today to learn more about how our customized lyophilized bead services can take your research to the next level!

 

Viewpoint

Please find below the link of our viewpoint on the isothermal amplification industry, which is invited by APAC CIO Outlook, a digital and print magazine that identifies and profiles emerging companies providing cutting-edge solutions to enterprises in APAC.

SBS Genetech: Leading the Next Generation Isothermal Amplification Technology

 

 

Only for research and not intended for treatment of humans or animals

 
 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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