Bst P DNA/RNA System has the following performance:

1. The whole Bst P DNA/RNA System includes hot start Aptamer, which ensures that the enzyme activity blocking efficiency is >95% at <30°C, and the enzyme activity is completely released within 1 min at >60°C. This characteristic facilitates the establishment of the reaction system at room temperature and greatly reduces the non-specific amplification at low temperatures.
2. The reaction temperature is further raised to 70°C, which greatly reduces the formation of primer dimer, improves the amplification specificity, and makes the nucleic acid release of crude samples more sufficient.
3. The whole portfolio contains Helicase, so Premium LAMP amplification (pLAMP) is allowed without using F3/B3 primers. At the same time, Helicase has the function of assisting in strand unwinding, which further reduces the concentration of FIP/BIP primers. This will further reduce non-specific amplification and greatly improve amplification homogeneity.

Comparison

Storage Conditions

For long-term storage, please keep it below -20°C (valid for 12 months). The repeated freezing and thawing of the product for 10 times will not affect the performance, but repeated freezing and thawing should be avoided.

Due to the high concentration of sugar components in the products, the enzyme stored at -20°C may have crystals when it is melted. At this time, 2.5× PrimeIAmp™ HotStart Bst P Basic MasterMix can be completely melted at 37°C. However, if Bst P DNA/RNA Polymerase is melted at 30°C, a high temperature may lead to the reduction of hot start performance. Once melted, it is recommended to store the product at 2-8°C. Under this condition, the product can be stored stably for 6 months.

Instruction: Protocol

Related: Bst P DNA/RNA Polymerase

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