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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
  • Contact
  • …  
    • Home
    • Product 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Natural Compounds
      • Synthetic Biology
      • Enzymes
    • POCT Solution 
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Freeze-Drying System
      • Lateral Flow System
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Beijing SBS Genetech Co.,Ltd.
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pBM16K Topo Cloning Kit

pBM16K Topo Cloning Kit

$176.00 - $460.00
pBM16K Topo Cloning Kit utilizes the inherent biological activity of DNA topoisomerase I. Topoisomerase can cleave and rejoin supercoiled DNA ends and therefore can be used to insert DNA fragments into vectors.
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All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: PBM16K-20 (for 20T)

Cat. No.: PBM16K-60 (for 60T)

 

Description

pBM16K Topo Cloning Kit is not only suitable for cloning blunt-end PCR products which are amplified by high-fidelity DNA polymerases such as Pfu, sPfu, KOD, Xerox, Phusion, Q5, and SuperGold™, but also can be used to clone PCR products with an extra "A" nucleotide at the 3'end which are amplified by DNA polymerases such as Taq, Taq plus, Tth and klenTaq. The pBM16K vector in the kit is linearized. Positive clones can be identified by colony PCR with primers M13F and M13R.

 

Features

  • The ligation reaction takes only 5 min.
  • Suitable for both blunt-end and A-tailed PCR products.
  • The vector adopts a new preparation process with zero background and no need for blue-white spot screening.
  • There are Sma I and EcoR V restriction sites on both sides of the cloning site, which is suitable for mono-restriction endonuclease analysis.
  • The vector carries the kanamycin resistance gene.
 
Instruction: Protocol
 
Related: pBM16A Topo Cloning Kit, pBM23 Topo Cloning Kit
 
 
Only for research and not intended for treatment of humans or animals
 
 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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