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for Superior Biology Services

  • Home
  • Products 
    • All Products
    • COVID-19
    • RNA-Related​
    • Custom Oligos Synthsis
    • Custom Peptides Synthesis
    • Nucleic Acid Purification​
    • PCR-Related​
    • Nucleic Acid Stain
    • DNA Markers
    • Genetic Manipulation
    • Biochemicals​
    • Nuclease
    • CRISPR Gene Editing​
    • Lab Supplies​
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    • About SBS
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      • All Products
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      • RNA-Related​
      • Custom Oligos Synthsis
      • Custom Peptides Synthesis
      • Nucleic Acid Purification​
      • PCR-Related​
      • Nucleic Acid Stain
      • DNA Markers
      • Genetic Manipulation
      • Biochemicals​
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    pBM23 Topo Cloning Kit
    pBM23 Topo Cloning Kit
    176.00 - 460.00
    pBM23 Topo Cloning Kit utilizes the inherent biological activity of DNA topoisomerase I. Topoisomerase can cleave and rejoin supercoiled DNA ends and therefore can be used to insert DNA fragments into vectors.
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    Cat. No.: PBM23-20 (for 20T)

    Cat. No.: PBM23-60 (for 60T)

     

    Description

    pBM23 Topo Cloning Kit is not only suitable for cloning blunt-end PCR products which are amplified by high-fidelity DNA polymerases such as Pfu, sPfu, KOD, Xerox, Phusion, Q5, and SuperGold™, but also can be used to clone PCR products with an extra "A" nucleotide at the 3'end which are amplified by DNA polymerases such as Taq, Taq plus, Tth and klenTaq. The pBM16K vector in the kit is linearized. Positive clones can be identified by colony PCR with primers M13F (-47) and M13R (-48).

     

    Features

    • The ligation reaction takes only 5 min.
    • Suitable for both blunt-end and A-tailed PCR products.
    • The vector adopts a new preparation process with zero background and no need for blue-white spot screening.
    • There are Sma I restriction sites on both sides of the cloning site, which is suitable for mono-restriction endonuclease analysis.
    • The vector has SP6 and T7 RNA polymerase promoter sequences on both sides of the multiple clone site, which can be used for in vitro RNA transcription.
    • The vector carries both ampicillin and kanamycin resistance gene.
     
    Instruction: Protocol
     
    Related: pBM16K Topo Cloning Kit, pBM16A Topo Cloning Kit
     
    Only for research and not intended for treatment of humans or animals
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      Let's Create the Future of Life Science

      A More Cost-Effective Scientific Research Solution

       

       

    • Types of Products

      We provide optimized solutions for life science research

      RNA-Related​

      Ribonucleic acid (RNA), as a key material for genetic information transmission and cell regulation, has been extensively studied in molecular biology. Like DNA, RNA is assembled in the form of nucleotide chains; but unlike DNA, RNA exists in nature in the form of single-stranded folds rather than paired double strands. We provide RNasin (RNase inhibitor), M-MLV reverse transcriptase and Carrier RNA to provide a complete raw material solution for RNA research. miAnalysis™ series are designed for microRNA quantitative research. And VirusMag™ series are designed for the isolation of viral RNA/DNA or bacterial DNA.

      Custom Oligos Synthsis

      DNA synthesis is a technology that links deoxynucleic acids (adenine, thymine, cytosine and guanine) together to form DNA. As the cornerstone of modern molecular biology, DNA synthesis plays a pivotal role in the field of synthetic biology. In addition to standard oligos synthesis, we also provide scientific research services such as long oligos synthesis, phosphorothioate oligos (S-Oligo) synthesis, modified oligos synthesis, fluorescent oligos synthesis, and real-time quantitative PCR probes to meet your different needs.

      Custom Peptides Synthesis

      Peptides are synthesized by condensation reaction of carboxyl group of one amino acid with amino group of another amino acid and are widely used in various fields such as antibody preparation, drug development and polypeptide vaccine development. Each of our polypeptides is accompanied by reliable HPLC and mass spectrometry data, detailed synthesis reports are provided, and the products are sent in a lyophilized state. Experienced staff can assist users in designing peptide chains and make appropriate recommendations for different needs of users, such as antibodies, special markers, large-scale synthesis, etc. In addition, we also offer Custom PNA Synthesis with high quality.

      Custom Peptide Library

      A peptide library is a new technique for studying structure-function relationship for a protein. A peptide library contains a great number of peptides that have a systematic combination of amino acids. The peptide library provides a powerful tool for drug design, protein-protein interactions, and other biochemical as well as pharmaceutical applications. It also has wide applications in drug screening, target validation, epitope mapping, and vaccine development.

      Gene Synthesis​

      Gene synthesis is a technology that synthesizes genes by artificial methods, which is one of the means of gene acquisition. Compared with the acquisition of genes from existing organisms, gene synthesis does not need templates and is therefore not limited by the source of genes. We use unique gene synthesis design software, which includes a full set of tools to design ideal structural units, thus enabling rapid and efficient gene construction and synthesis in a single reaction. Please do not be limited by restriction sites and polylinkers, we will synthesize the various gene sequences you need.

      Nucleic Acid Purification​

      Nucleic acid purification is an important component of molecular biology and has a wide range of applications in medicine and biological sciences. Our nucleic acid purification kit uses first-class silica gel column technology (SiMax™ Spin Column, SiMax™ Genomic DNA Extraction, SiMax™ PCR Products/Agarose Gel Purification, SiMax™ Plasmid DNA Miniprep) and magnetic beads technology (VirusMag™ Magnetic Beads, VSep™ Magnetic Separators, VirusMag™ One-Step DNA/RNA Isolation Kit, VirusMag™ DNA/RNA Isolation Kit), which can purify DNA from various sources quickly and reliably. The purity of DNA purified by our kit is very high and it is suitable for many downstream applications such as sequence determination, cloning and cleavage.

      PCR-Related​

      Polymerase chain reaction (PCR) is a widely used method in molecular biology, which can rapidly replicate millions to billions of specific DNA samples, enabling scientists to extract only a small amount of DNA samples for detailed research. We provide a variety of DNA polymerases (Taq, Bst, f-Pfu, sPfu) and corresponding PCR premixes, covering a wide range of scenarios such as high fidelity, high specificity, and rapid amplification. Scarlet™ series kits provide a perfect solution for blood direct PCR, and PrimeSNP™ Genotyping Kit uses Competitive Allele Specific PCR method for genotyping of purified DNA samples. High quality dNTPs (set, mix) are also supplied.

      DNA Markers

      DNA markers are used to determine the approximate size of molecules on the gel during electrophoresis, based on the principle that the molecular weight is inversely proportional to the mobility through the gel matrix. We provide abundant DNA molecular weight standards that can be used for various fragment lengths. Our fragments of DNA markers have been purified separately by proprietary technology, so their quality is superior to industry standards.

      Genetic Manipulation

      Gene manipulation is a process that uses biotechnology to manipulate genes directly to generate new DNA, and has been widely used in research, medicine, industrial biotechnology and agriculture. Our Premium™ Master Assembly Mix, Muta-direct™ Site-Directed Mutagenesis Kit and Topo Cloning kits (pBM23, pBM16A, pBM16K) provide efficient solutions for these types of demands.

      Nucleic Acid Stain

      GoodView™ is a safer nucleic acid stain, an alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 268 nm and another at 294 nm. In addition, it has one visible excitation at 491 nm. The Fluorescence emission of GoodView bound to DNA is centered at 530 nm. Our GoodView™ Nucleic Acid Stain is also included on New Products, Science Magazine, January 11, 2019.

      Biochemicals​

      Biochemistry, as its name implies, is a discipline that studies chemical processes in organisms, often referred to as biochemistry. It is mainly used to study the structure and function of various intracellular components, such as proteins, sugars, lipids, nucleic acids and other biomacromolecules. We provide variety types of high quality biochemical reagents to meet these demands, including Proteinase K, Mutant Proteinase K, Besta™ LE Agarose, IPTG, X-Gal, DNase I (RNase Free), Enterokinase, SUMO Protease, TEV Protease, PreScission Protease (PSP), Bovine Serum Albumin, T4 DNA Ligase, etc.

      Nuclease

      A nuclease is an enzyme capable of cleaving the phosphodiester bonds between nucleotides of nucleic acids. Nucleases variously effect single and double stranded breaks in their target molecules. Here we offer various high quality nucleases, including RNase A, RNase H, Thermostable RNase H, Benzonase Nuclease, Uracil N-Glycosylase, T7 Endonuclease I, T5 Exonuclease, Shrimp Alkaline Phosphatase, Terminal Transferase, etc.

      CRISPR Gene Editing​

      CRISPR gene editing is a genetic engineering technology in molecular biology that is used to modify the genome of an organism. By introducing Cas9 nuclease into cells with a guide RNA (gRNA) complex, specific locations of the cell genome will be cleaved so that existing genes can be removed and/or new genes added in vivo. We provide high quality chemically modified Synthetic sgRNAs to provide a more reliable solution for CRISPR gene editing.

      Lab Supplies​

      We provide various high quality common lab supplies, including ELISA Plates, Cell Culture Dishes, Cell Culture Plates, Microcentrifuge Tube, Centrifuge Bottles, Centrifuge Tubes, PCR Plates, PCR Tubes, Pipette Tips, Serological Pipettes, 96-well Deep-Well Plate, Conical Cryovials, SiMax™ Spin Column, with high cost-effective ratio to make your experiments smoother!

    • Best Sellers​

      Proteinase K

      RNase and DNase free

      Thermo-stable M-MLV Reverse Transcriptase

      Synthesize the complementary DNA strand from a single-strand RNA template

      SiMax™ Spin Column

      Silica membrane based spin column for DNA/RNA extraction

      Bst DNA/RNA Polymerase

      Suitable for isothermal amplification of RNA

      PrimeTaq™ Probe One-Step RT-qPCR Kit

      Ultra-high sensitivity quantitative detection of RNA

      VirusMag™ DNA/RNA Isolation Kit (100 preps)

      Automated-ready kit for virus RNA extraction

    • Why Choose Us?

    • Reliable Global Supply Chain

      With reliable GSC, we are recognized as one of the leading APAC biotech B2B suppliers.

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      Trusted by Top Researchers

      Our high quality products are trusted by researchers and scientists in top universities and institutes.

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      Powerful Technical Support

      Our support personnel are all experts with average more than 10 years of working experience.

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    • SBS Genetech is a long-term sponsor of

      Cold Spring Harbor Laboratory

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    • Contact

      For more information, please fill out the provided form or contact us directly by E-mail

      tech@sbsbio.com
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